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JM Science
JM Science

Sample Prep for Chromatographic Analysis

By | April 6 2012

Balancing the needed increases in selectivity with the desire for generic methods The Greek translation of chromatography— basically “color writing”— fails to reveal the breadth of this technology. The range of

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Thermo Fisher
Thermo Fisher

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  It’s official: Sample and standards preparation are significant bottlenecks in high-throughput laboratories, particularly those partly or fully automated. Improved instrument sensitivity—and the need for speed and accuracy—have made sample prep a top priority.

A Question and Answer with Select Automated Sample Prep Experts

Despite protestations to the contrary, automation is not something to undertake casually. Due to the dizzying array of possibilities and workflows, the technology has not yet achieved the simplicity of consumer products.

A comprehensive review of sample preparation would require a multivolume work. Beyond that, covering the possible number of workflows given the hundreds of potential “unit operations” would fill an encyclopedia, especially when solids (rock, soil, animal tissue) are considered.

Sample preparation (“prep”) is a tedious, time-consuming task but a necessary part of nearly every analytical workflow, regardless of industry or laboratory type.

Arguing against lab automation is becoming more difficult, particularly for sample prep. With reagents and solvents costing what they do, a business case based on direct cost savings for critical materials stands on its own.

PBI Shredder SG

  • A good fit for the extraction of DNA, RNA, protein, mitochondria, and small molecules
  • Creates a closed system in which to safely prepare samples
  • Portable for easy and efficient field collection
  • Operates by using pressure, which forces the sample against the lysis plate in the tubes for low-shear cell disruption

Cole-Parmer
www.coleparmer.com

Microlute™ SPE

  • Provides a faster, trouble-free alternative to cartridges for high throughput sample clean-up
  • Handles samples as small as 150ul
  • Includes filter plate, collection plate, waste tray and manifold
  • Offers all the advantages of automated and high throughput SPE sample preparation in a convenient microplate format

Porvair Sciences
www.porvair-sciences.com

Laboratory blender ideal for both sample prep and small scale production of topical therapeutic and cosmetic products.

Microwave digestion is the preparation method of choice for analyzing metals in complex mixtures. Food and environmental industries favor this technique, which is also applicable to testing materials, soil, agricultural waste products, engine oils,

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Problem: Highly concentrated nucleic acid and protein samples must be diluted before they can be read on most absorbance-based spectrophotometers, and conversely, diluted samples must be concentrated

Most Recent

PBI Shredder SG

  • A good fit for the extraction of DNA, RNA, protein, mitochondria, and small molecules
  • Creates a closed system in which to safely prepare samples
  • Portable for easy and efficient field collection
  • Operates by using pressure, which forces the sample against the lysis plate in the tubes for low-shear cell disruption

Cole-Parmer
www.coleparmer.com

Microlute™ SPE

  • Provides a faster, trouble-free alternative to cartridges for high throughput sample clean-up
  • Handles samples as small as 150ul
  • Includes filter plate, collection plate, waste tray and manifold
  • Offers all the advantages of automated and high throughput SPE sample preparation in a convenient microplate format

Porvair Sciences
www.porvair-sciences.com

Most Recent

In this free, educational webinar brought to you by Lab Manager Magazine a panel of technical experts representing leading vendors will discuss some of the trends and improvements in dealing with sample preparation for MS.

In this free, educational webinar brought to you by Lab Manager Magazine, a panel of technical experts representing leading vendors will discuss some of the trends and improvements in dealing with sample preparation for chromatography.

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Exeter Analytical has developed a reliable sample preparation technique that enables accurate determination of the percentage Carbon (C), Hydrogen (H) and Nitrogen (N) in difficult to analyze volatile samples.

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Aqua Solutions
Aqua Solutions
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W.S. Tyler
W.S. Tyler

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