Original broadcast date: Tuesday July 27, 2010
Dr. Gregory Shipley, director of the Quantitative Genomics Core Laboratory at The University of Texas Health Science Center in Houston, discusses with Tanuja Koppal, contributing editor to Lab Manager Magazine, the ways in which he goes about evaluating and investing in new technologies and reagents for carrying out real-time quantitative polymerase chain reactions (qPCR) for the various assays being conducted in his core facility. Dr. Shipley is an expert in designing qPCR assays and has written on the topic in various scientific journals and spoken on it at several conferences.
Dr. Shipley offers valuable insights on how to apply real-time qPCR instruments by taking cell lysates from a small sample or from a single cell to perform RT-PCR, without having to isolate the RNA.
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