Thermo Fisher Scientific is pleased to announce a new method that uses high-performance liquid chromatography (HPLC) combined with charged aerosol detection (CAD) to enable improved quantitation of triterpene content in gotu kola. Low-wavelength UV detection commonly used for triterpene analysis often results in strongly sloping baselines, and interferences from minor components can adversely affect quantitation. Customer Application Note (CAN) 111: Determination of Triterpenes in Centella asiatica (Gotu Kola) by HPLC-CAD demonstrates that charged aerosol detection minimizes these interferences and improves the baseline, thereby enabling improved quantitation of triterpenes in gotu kola.
Gota kola is a small herbaceous annual plant used as a medicinal herb in Ayurvedic medicine and used in traditional Chinese medicine to treat a wide variety of conditions including improvement of memory and blood flow. Gota kola is also used as a topical application for skin conditions such as ulcers, wounds, and eczema. The chemical compounds of interest in gotu kola are usually considered to be the ursane- and oleanane-type triterpenes and the triterpene glycosides including asiaticoside, madecassoside (asiaticoside A), asiaticoside B, madecassic acid, asiatic acid, and terminolic acid.