Volume 7 Issue 7 | August 2012
Atomic absorption (AA) has been known since the 19th century, but it was not until the 1950s, thanks to efforts by Alan Walsh at Australia’s CSIRO research center, that use of AA spectrometers became routine for metals analysis. Four Fast
Chromatography Analysis Sample Prep
Sample preparation is an essential step in many analyses and the importance of accurate sample preparation techniques cannot be overstated. The sample preparation procedure significantly impacts assay throughput, data quality, and analysis cost
The right chromatography data system (CDS) can spell the difference between a streamlined data collection process and one that is haphazard. CDSs are software packages that collect, store, and help users interpret data. Some have instrument control
In flame photometry, a branch of atomic spectroscopy also called “flame atomic emission spectrometry,” atoms are examined in the spectrometer. This technique is suited to the quantitative and qualitative determination of a variety of
Fourier transform infrared (FTIR) spectroscopy, a subset of infrared (IR) spectroscopy, uses a mathematical algorithm, Fourier transform, to translate raw infrared data into a spectrum. FTIR is useful for the analysis of organic and inorganic
Gas chromatography (GC) was at one time commonly called “GLC,” where the “L” stands for liquid. Inside GC columns are particles of a ceramic or inert material coated with an extremely viscous liquid stationary phase that
Gas chromatography-mass spectrometry (GC-MS) is the fastest-growing GC method. Mass detection, which can take a variety of forms based on the MS component, provides a dimension that conventional thermal conductivity or flame ionization detectors
Chromatographers who learned their craft 20 years ago may not have been familiar with autosamplers then, but today nearly every high performance liquid chromatography (HPLC) instrument includes an autosampler as standard equipment. Top 5
In HPLC columns, reverse-phase and normal-phase chromatography separation methods are based on polarity. Reverse phase columns separate analytes based on their hydrophobicity, with the more hydrophobic compounds being retained longer on the column.
UPLC Screening to Preparative HPLC Using CSH Column Technology and Focused Gradients
INTRODUCTION Reaction screening and purification are essential steps in small molecule pharmaceutical discovery labs. Cleaner samples for subsequent synthesis can reduce reaction by-products and facilitate the critical final purification of
Top 6 Questions You Should Ask When Buying hplc sample prep products (SPE): 1. What is your sample matrix? 3. What are your target analyte(s)? 4. What are your sample clean up goals (high recovery vs. clean-up vs. speed)? 5. What
HPLC is, for many scientists, an essential apparatus for the separation, identification, purification and quantification of various compounds. Users of HPLC work in a variety of fields including biomedical research, and the cosmetics, energy, and
Smartprep Spe Cartridge System: Automatic Sample Volume Calculation
Analytical methods which extract aqueous samples require that the initial volume of the sample be determined, as it is necessary to calculate the concentration of the analytes in the sample. Typically, this is done by marking the sample meniscus on
Mass Spectrometry (stand alone/integrated)
Top 6 Questions You Should Ask When Buying a mass spectrometer 1. What factors come into play when determining the MS specifications you require in terms of throughput, sensitivity, robustness, software control, ease of use, and ease of
Integration is the main reason for the virtual disappearance of the specialist GC or MS supplier that produces chromatographs for someone else’s mass detector, and vice versa. The level of component and software interoperability demanded by
Time-of-flight (TOF) mass spectrometry (MS) works by imparting equal energies to all fragments. Since smaller particles thereby travel faster, their masses are determined by how quickly they travel from the source to the detector.
The technology behind visible light microscopes, the oldest true laboratory instruments, has not changed much in fundamental operation in 200 years. Microscopes still consist of a light source, optics, and a stage for holding the specimen. But that
Recent Trends in Particle Size Analyzers • Users are working with smaller and smaller particles. • Customers are demanding equipment that’s reasonably priced and that takes measurements rapidly, with a high degree of
Recent Trends in Raman Spectrophotometers • Once massive benchtop instruments that required a Ph.D. to operate, portable Raman spectrophotometers are now a booming business, with the latest instruments being more approachable for
Top 5 Questions You Should Ask When Buying a uv-vis spectrophotometer 1. What applications will you be using the instrument for? This will help you determine the detection range you require. Don’t forget to consider future applications
Modular Spectroscopy Tools for the Simultaneous Measurement of Multiple Sample Characteristics
Modular spectrometers and accessories can be combined for measurement of various spectral characteristics. In this application note we describe a spectrometer and multifunction accessory appropriate for measurement of absorbance, fluorescence,
Measurement of Gold Nanoparticle Size and Concentration by Spectrophotometry
Although gold nanoparticle production can be controlled to yield specific size ranges, both the concentration and size of nanoparticles must be checked following production. UV-Vis spectrophotometry is an established QC method for this; however
Top 6 Questions You Should Ask When Buying a Lab Balance 1. What is the heaviest sample you will weigh (including container weight) and what is the lightest sample? 2. What is the required +/- tolerance of your lightest sample? 3. How many
Adam Equipment’s PMB 53 Moisture Analyzer Clearly Shines In Glass Manufacturing Applications
It has become crystal-clear to most of us that glass is something we just can’t live without. Scientists use glass beakers for measuring chemicals. We wear eyeglasses to improve our vision or to make a fashion statement. Glass windows protect
New Design • In 2Y series cable was hidden under the main housing now is folded behind the indicator • New bottom design of AS/3Y and PS/3Y. Operating System – Windows CE New operating system enables better communication
Biological safety cabinets (BSCs) are enclosures that protect users and the environment from biohazards by removing particulates and aerosolized pathogens from the work area through HEPA filtration, then recirculate or exhaust the purified air, hence, cleansing the workspace air.
ERGO: “WORK” NOMOS: “THE STUDY OR LAWS OF” COMBINES TO MEAN: “THE LAWS OF WORK” OR DESIGNING “EQUIPMENT” THAT ACCOMMODATES LIMITS OF THE USER Advancements for Improved Safety Conditions –
Welcome to a comfortable clean bench
The AniGARD e3 animal transfer station from The Baker Company combines improved containment and enhanced ergonomics with lower energy requirements to provide you with unrivaled performance and protection. Comfortable and Versatile to Increase
Centrifuges separate particles and structures suspended in liquid by applying thousands of gravitational force equivalents to the sample through spinning. Laboratories use centrifuges to clarify suspensions, separate liquids, isolate suspended
New Mini centrifuge with BIG benefits
Labnet International is proud to introduce their new personal mini centrifuge, the PRISM MINI. Reliable and easy-to-use, the Prism Mini is compact and economical, allowing each workstation to be equipped with a “personal” centrifuge. The
Water baths are used in industrial and clinical laboratories, academic facilities, government research laboratories, environmental applications, as well as food technology and wastewater plants. Chillers, on the other hand, are refrigerators that
Thermo Scientific Liquid Temperature Control
Highlighting innovative design features and useful application information It is a common misconception that a bath with a larger reservoir provides greater stability because the addition of heat energy results in a smaller temperature rise,
A cleanroom is a rigorously controlled environment that has a low level of environmental pollutants such as dust, airborne microbes, aerosol particles and chemical vapors. The air entering a clean room is filtered and then continuously circulated
Cell culture incubators, also known as carbon dioxide (CO2) incubators, are by far the largest group in terms of sales and applications. CO2 incubators support cell culture work in basic research and in the biotechnology industry where CO2 is used
Contamination Prevention Without Compromise
Protect your valuable samples with Thermo Scientific 100% pure copper CO2 incubators The Thermo Scientific 100% pure copper CO2 incubators allow you to culture precious samples, without worry. The 100% pure copper antimicrobial interior prevents
BINDER Inc. offers a range of environmental simulation equipment that provides consistent performance – a quality that allows a focus on the research at hand and confidence in the equipment holding samples. CO2 Incubators, Environmental
ELNs are not the hub for capturing every piece of laboratory data. There will always be a need for LIMS, SDMS and other informatics repositories that are specifically designed to handle structured or unstructured information. ELNs are, however,
Accelrys Electronic Lab Notebooks
Accelrys has a range of Electronic Lab Notebooks (ELN) to meet the full spectrum of needs of scientific organizations from research to development to manufacturing. By improving lab-to-market operations by moving from paper to electronic workflow
Evaporators & Evaporation Systems
Rotary evaporators have for decades been staples in labs and industries performing chemistry, including labs in the chemical, environmental, materials, life science and forensics industries. Key applications include sample concentration, solvent
Using the CentriVap® Micro IR for Genomic DNA Isolation
Processing DNA samples can be a time consuming and expensive process. If degradation occurs during any of the steps, the process must start over. Before you begin, make sure that the DNA is free of RNA and protein contamination. To prevent
CentriVap® micro IR Vacuum Concentrators by Labconco
With the smallest footprint available, the new CentriVap micro IR Vacuum Concentrator fits in tight spaces and is ideal for small throughput in molecular biology, proteomics, genomics, genetics, cell biology and drug discovery labs. Weighing in
As a mature market, filtration moves forward incrementally through improvements in filter media and housings. Improvements in lab instrumentation and techniques demand a new level of sample preparation that was unnecessary a decade ago.
Flow cytometry is a powerful technology that allows researchers and clinicians to perform complex cellular analysis quickly and efficiently by analyzing several parameters simultaneously. Top 5 Questions You Should Ask When Buying a Flow
The use of freeze dryers extends from applications in academic labs to zoos. Moreover, this technology contributes to basic research and manufacturing. For example, scientists at a zoo might use a freeze dryer to increase the concentration of a
One of the primary safety devices in laboratories where chemicals are used is the laboratory fume hood. A well-designed fume hood, when properly installed and maintained, can provide a substantial degree of protection for the researcher, provided
AirClean® Systems Independence™ Ductless Fume Hood
As research and laboratories evolve, AirClean® Systems continues to innovate with sophisticated, efficient fume containment solutions. Independence™ is the culmination of two decades of research and development in airflow design, gas-phase
If you’ve had to lug a tank of gas to an experiment station and secure it in place, you know the hassle and danger involved. Many lab managers are turning to generators for convenience; cost also plays a part with labs needing to pay for the
Glove boxes are specialty enclosures that allow tight control over experimental conditions. Sizes range from less than five to several hundred cubic feet. Glove boxes maintain temperature, humidity, and process gas while protecting the experiment
Homogenizers: 6 Questions to Ask When Buying a Homogenizer
Turning a sample into a suspension—the essence of homogenizing— occurs in a wide range of laboratory applications. In life science and clinical research, scientists often homogenize tissue samples for various analytical studies. Top
The question of limited access or general access to laboratory goods and services applies to a range of instruments, utilities, and competencies. These questions take on added significance for midsized or larger labs. Whether to employ central
LIMS: Laboratory Information Management Systems
A Laboratory Information Management System (LIMS) serves as the interface to a laboratory’s data, instruments, analyses and reports. For many analytical laboratories, a LIMS is an important investment that assists management in evaluating the
Low-temperature laboratory freezers will achieve temperatures of -40°C to -85°C, much lower than their kitchen-based counterparts. When selecting a freezer or refrigerator for your lab, it is important to know what specific storage
Microwave digestion is the preparation method of choice for analyzing metals in complex mixtures. Food and environmental industries favor this technique, which is also applicable to testing materials, soil, agricultural waste products, engine oils,
Microwave-Assisted Extraction of Semi-Volatile Organic Compounds Using USEPA Method 3546
Conventional techniques for environmental organic sample preparation for priority pollutants, such as extractions by shakers, Sohxlet, or sonication, are time-consuming and generate large volumes of solvent waste. In addition, workers are exposed
In a laboratory, most materials required for sampling are, in practice, nonhomogeneous mixtures. The best method of obtaining a small representative sample of the nonuniform whole is to take a quantity of the material large enough to be
Nobody gets excited about lab ovens, but they are definitely essential lab components. Ovens are found in almost every industrial, research and development laboratory. Applications include drying lab ware and keeping it ready for use; sterilization;
The expanded features in today’s pH meters reflect the broad use of this technology. Researchers use pH meters in a wide range of research fields—including biological and chemical, agricultural and environmental, and more—and
Best practices dictate that pipettes undergo preventive maintenance and calibration at least once per year. Calibration involves dispensing set volumes of a liquid, usually water, into the weighing pan of a calibrated balance. Service personnel
Simply Better Pipetting: NEW! Eppendorf Xplorer plus
Eppendorf presents its new electronic pipette, the Eppendorf Xplorer plus®. The intuitive operating concept and ergonomic display of our Xplorer set new standards in simplicity, precision and reproducibility. The Eppendorf Xplorer is specially
Power supplies provide steady, precisely controlled electrical energy to electronic equipment. Anyone who has built or worked with a desktop computer recognizes the power supply— typically the bulkiest component—sitting behind the
A refractometer is an optical instrument that is used to determine a concentration of a particular substance within a given solution. The measure of how much the speed of light is reduced when traveling through a substance such as a gas, solid, or
Freezers and refrigerators are an integral part of any laboratory that requires temperature controlled storage. Maintaining samples at an optimal temperature while in storage is vital for many lab professionals and this makes choosing the right
Nor-Lake® Scientific -86ºC Select™ Ultra-Low Upright Freezers
Designed to meet the demanding requirements for scientific and laboratory research. Advanced engineered design incorporates the latest in cabinet, refrigeration, temperature control and monitoring features. Provides energy efficient, convenient,
Lab shakers are available in two different mixing actions: orbital and reciprocal and are primarily used for culturing organisms in shake flasks or test tubes, and can also be used for mixing liquids. Shakers range from small to mid-size bench top
Specialty gases have become critical components for nearly every industry, including chemicals, biotechnology, materials processing, environmental, foods, semiconductors, and medical. Defining the term “specialty gas” seems simple at
Magnetic stirrers are a popular type of laboratory stirrer that use a rotating magnetic field to cause a stirrer bar to rotate within the solution. These stirrers are often combined with a hotplate and are ideal for small volumes of non-viscous
Pharmaceutical and polymer industries are increasingly interested in both TGA (thermogravimetric analysis) and DSC (differential scanning calorimetry). Drug companies use these techniques to test for drug stability and crystalline state (or lack
Titrators are specialized instruments that perform titrations with minimal operator intervention and can thus minimize errors, improve throughput, and facilitate documentation. There are two major titrator types: potentiometric acid-based designs
A mainstay of environmental and quality control chemistry, TOC analyzers measure the carbon content of dissolved and particulate organic materials in water. The carbon measured may arise from any combination of living or dead organisms and chemical
Vacuum pumps are an essential piece of equipment and are used in a wide variety of processes in most laboratories. Over the past 25 years, vendors have made significant innovative improvements to vacuum pumps, with important developments in high
Economic Advantage of Oil-free Vacuum for Labs
Abstract: Oil-free vacuum pumps can offer significant scientific and economic advantages over oil-sealed rotary vane pumps for most lab applications. Forearm Support for Comfort and Safety Introduction Most lab managers appreciate the
Viscometers measure viscosity, the resistance of fluids to flow or stress. In common terms, viscosity is related to a fluid’s “thickness”—a physical property of great interest to manufacturers of liquids, slurries, and pastes
A successful water system design begins with a clear and precise definition of user needs throughout the facility. The purity level and volume of water required at each point of use can vary considerably and therefore must be fully assessed in order
Maximizing UV light for Lab Water Systems
Abstract: Conventional lab water systems use UV light by flowing water in a chamber around the bulb in a protective quartz sleeve as part of the recirculation loop. Using innovative technologies, the UV light benefits are maximized but sterilizing
PURELAB flex- innovating water purity
ELGA’s new innovative Type I ultrapure water purification system ensures accurate consistent results. The PURELAB flex 3 & 4 are the latest additions to the award winning PURELAB flex range of systems. Both systems deliver up to 10 liters
Top 7 Questions You Should Ask When Buying an automated liquid handling system 1. What kind(s) of dispensing technology is used? Peristaltic pump dispensing offers low prime volumes and back-flushing; while microprocessor- controlled syringes
Hamilton is a global leader of world class analytical components, medical instrumentation, temperature control systems, laboratory robotics and automated liquid handling equipment. From Clark Hamilton’s development of the Microliter Syringe in
Accelerating 3D Microtissue Production
Preclinical drug development relies on advanced in vitro models with a high degree of standardization to achieve maximal predictivity for an efficient compound de-risking strategy. InSphero’s scaffold-free organotypic three-dimensional (3D)
Eppendorf epMotion 5075: Automation made simple
The epMotion 5075 LH is the ideal solution for advanced liquid handling demands. It offers outstanding accuracy and precision making it an excellent tool for demanding, small-volume applications such as real-time PCR set-up or magnetic bead
BRAND® Titrette® Bottletop Burette Delivers Class A Precision
The BRAND Titrette® is the world’s first bottletop burette to provide titration within ASTM and ISO error limits for Class A glass burettes. This precision is achieved through a novel magneto-resistive measurement system developed by BRAND
The GX-241 Liquid Handler is a compact workhorse that provides the perfect balance of size, performance, and capabilities to individual labs. Designed for applications and labs where bench space is at a premium, it features a large injection volume
Automate your Buffer Preparation with the Tecan Buffer Creation Wizard The Buffer Creation Wizard is a Freedom EVOware® Add-on and allows the fully automated preparation of your set of buffers e.g. for subsequent screening experiments like
Mixing solutions is one of the most common laboratory tasks. Over the years, a number of automated methods for mixing have been devised, all of which remove this burden from the operator by offering a sustained and controlled stirring action for
Electrophoresis relies on a basic process—particles moving in an electric field, more or less. Although known for more than 200 years, this phenomenon still drives fundamental techniques in many laboratories. Despite that long history, this
Microplate handlers are specialized robotic devices that transfer microtiter plates in three-dimensional space from one location within a workflow to another. The “locations” are actually operations such as solvent addition
Microplate readers are widely used in research, drug discovery, bioassay validation, QC, and manufacturing processes for the detection of biological, chemical, or physical processes in samples contained in microtiter plates. Top 8 Questions You
The Array Tape™ Platform Think outside the plate!
The platform instrumentation from Left to right: Araya®, Soellex® and Nexar® The Array Tape™ Platform is the new standard for high throughput applications. Achieve quality data with repeatable results in a flexible, high
Products in Action: PersonalArrayer 16
Core Life Sciences has partnered with CapitalBio Corporation, a leading developer of microarray technology, to offer the PersonalArrayer 16, a new highly flexible and efficient microarrayer suitable for a wide range of applications. The
Synergy™ NEO HTS Multi-Mode Microplate Reader
Synergy NEO is BioTek’s newest development in multi-mode microplate readers. Designed specifically for today’s screening and core laboratories, NEO has all the features you would expect from an HTS instrument, including multiple parallel
Within a decade of its discovery in 1983, Real-Time PCR—also called quantitative polymerase chain reaction (qPCR)—evolved into one of the most powerful and sensitive gene analysis techniques available. Real-time PCR measures PCR
Reagent companies sell RNAi reagents as “virtual kits” consisting of the shRNA or siRNA sequences and an appropriate transfection reagent. Vendors usually guarantee that a certain percentage of multiple knockdown constructs they sell for
This year marks the 25th anniversary of commercial thermal cyclers for the polymerase chain reaction (PCR). In brief, this technology amplifies segments of DNA. In such a device, PCR tubes go in holes in a thermal block.
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