In HPLC columns, reverse-phase and normal-phase chromatography separation methods are based on polarity. Reverse phase columns separate analytes based on their hydrophobicity, with the more hydrophobic compounds being retained longer on the column. Separations based on charge utilize ion-exchange chromatography.
Top 7 Questions You Should Ask When Buying HPLC Columns:
1. Based on your analyte(s), matrix, separation goals, and instrumentation, what column does the vendor recommend?
2. What benefits does this column offer over your current column? Performance? Lifetime? Reproducibility? Etc.
3. How should you clean/prepare your sample prior to injection on the column?
4. How do you care for the column? Conditioning, cleaning, storage, etc.
5. What type of chromatographic media (fully porous, monolithic, core-shell) is going to provide the most benefit for your separation?
6. Do you need a unique selectivity (HILIC, polar-end capped, etc.) to separate any very polar and/ or non-polar components in your mixture?
7. What column dimension is going to be most suitable for your loading requirement?
Based on our latest HPLC columns survey, reverse-phase is the most popular method in our readers’ labs.
|Hydrophilic interaction (HILIC)||7%|
|Gel permeation (GPC)||7%|
|Gel filtration (GFC)||5%|
Analytical-scale columns are the most common scale of column used by our readers.
|Narrow-bore (1 to 2 mm diameter)||20%|
|Large ID (>10 mm diameter)||11%|
The most important feature for our surveyed readers when they are buying HPLC columns is the technical performance of the columns.
|Technical performance of HPLC columns||19%|
|Shorter run times/increased throughput||12%|
|Lot-to-lot reproducibility of HPLC columns||10%|
|Ruggedness/durability of HPLC columns||9%|
|Lower operating costs (reduce solvent use and waste)||8%|
|Reputation of column manufacturer||8%|
|Purchase price of column||6%|
|Breadth of HPLC column offering (selectivity)||6%|
|Method validation/compliance support||3%|
|Safety and health features||1%|
Recently Released HPLC Columns
Kinetex® Core-Shell HPLC/UHPLC
• Meets researchers’ need to improve results and increase productivity without large capital expenditures
• Delivers significant improvements in speed and separation efficiency over traditional 3- and 5-micron columns
• Enables chromatographers to get performance comparable to sub-2-micron columns without investing in UHPLC systems
• Product line currently includes six phases with columns in 2.6- and 1.7-micron particle sizes
ZORBAX 300SB-C3 and 300-Diphenyl 1.8 μm
• Extend the benefits of reversed-phase biomolecule separations to ultra-high-performance liquid chromatography
• C3 phase broadens the selectivity and improves peak shape and recovery options for larger proteins
• Diphenyl phase offers additional selectivity
Accucore™ 150-C18 & 150-C4
• Designed to improve protein and peptide assay robustness
• Enhance peak capacity and run times at moderate operating pressures
• 150-C18 has been further optimized for biomolecules and protein digests
• Low hydrophobicity C4 ligand used in the Accucore 150-C4 column enables fast protein separations • Feature solid core particles engineered to an average size distribution of 1.12
Thermo Fisher Scientific
CILS-8200S Series HPLC Column Labels
•Designed to permanently adhere to all surfaces and withstand aggressive HPLC cleaning agents
• Solvent-resistant • Printable straight from a standard desktop printer
• Variable data (batch numbers, barcodes etc) can be added ‘at the click of a button’
HPLC Columns Manufacturers
|Advanced Chromatography Technologies||www.ace-hplc.com|
|Grace Davison Discovery Systems||www.discoverysciences.com|
|Thermo Fisher Scientific||www.thermoscientific.com|