Sample Prep for Chromatography

It’s official: Sample and standards preparation are significant bottlenecks in highthroughput laboratories, particularly those partly or fully automated. Improved instrument sensitivity—and the need for speed and accuracy—have made sample prep a top priority.

Yet discussing sample prep as a monolithic operation is impossible because prep steps, analyses, and matrices are highly variable among industries. Measuring protein levels in energy drinks is not the same as for granola; food safety analyzes for pesticide residues and microbial contamination; still other tests quantify trace nutrients. Matrix removal is a great idea, but even the “matrix” differs for all the tests mentioned.

Top 6 Questions You Should Ask When Buying HPLC Sample Prep Products (SPE)

		What is your sample matrix?
		What volume of sample do you need to extract?
		What are your target analyte(s)?
		What are your sample clean-up goals (high recovery vs. clean-up vs. speed)?
		What sample preparation format is best for your work?
		What is your mode of analysis (LC-UV, LC-MS-MS, GC-FID, GC-MS etc.)?

Top 6 Questions You Should Ask When Buying a System for Chromatography Analysis Sample Prep

	Can this system perform exhaustive extractions in about 10 minutes?
	Can this extraction system operate unattended overnight?
	Does the system change solvents and perform rinses automatically between samples?
	Does the extraction system comply with U.S. EPA methods?
	How does solvent consumption compare to other systems?
	Can the extraction system handle solid and semisolid and samples, or are multiple systems needed?

Recently Released Chromatography Sample Prep Products

Metrohm Combustion Ion Chromatograp hy System

• Automates the determination of halogens and sulfur of various matrices across different industries
• Complies with environmental requirements, including DIN, EN, IEC, RoHS, WEEE and more
• Flame sensor technology measures the light intensity from the pyrolysis oven, controls the feed rate of the sample boat and prevents soot formation
• Combustion by one universal method

Metrohm
www.metrohmusa.com

Strata ® and Strata -X Tabless Tube Holder Accessories

• Hold SPE tubes in 96-well spacing to integrate with automated systems
• All Strata and Strata-X products are now available in the tabless 1 mL tube format, enabling the user to arrange multiple sorbents within the holders
• Allows users to remove and replace a single SPE tube should they make an error

Phenomenex
www.phenomenex.com

Microlute™ SPE Sample Preparation System

• Provides a faster, trouble-free alternative to cartridges for high throughput sample clean-up
• Handles samples as small as 150ul
• Includes filter plate, collection plate, waste tray and manifold
• Offers all the advantages of automated and high throughput SPE sample preparation in a convenient microplate format
• Capable of rapidly processing 96 samples in one go repeatedly and precisely

Porvair Sciences
www.porvair-sciences.com

EVOLUTE® EXPRESS 96-Well Plates

• Simplify polymer-based SPE by employing a streamlined 3-step protocol; Load-Wash-Elute
• Reduce processing and method development times without loss of analyte recovery or method robustness
• Design provides a uniform flow in the processing of aqueous samples and eliminates the need to re-run samples due to well-clogging

Biotage
www.biotage.com

Sample Prep for Chromatography Manufacturers