What You Absolutely Must Know if You're Using a BSC

Anyone using a biosafety cabinet should be trained In at least the following.

  • The rationale for why the biosafety cabinet has been selected (sterility, biosafety, or a combination). This would include the risk assessment process that led to its selection for use.
  • Aseptic technique and good microbiological work practices.
  • Personal protective equipment (PPE) required for the proposed use of the biosafety cabinet, including how PPE should be donned and doffed.
  • How the biosafety cabinet operates and what are the potential interferences of successful operation.
  • How to use the biosafety cabinet, from turning it on, preparation for work, carrying out work, and how to terminate work and decontaminate the biosafety cabinet to prepare it for its next use.
  • Maintenance of the biosafety cabinet.
  • How to determine if the biosafety cabinet is operating adequately (checks of pressure gauges, odd noises, emanation of odors, etc.) and to whom they should be reported for investigation.

(Source: Karen Byers, President, American Biological Safety Association)

Categories: Laboratory Technology

Published In

Laboratory Etiquette Magazine Issue Cover
Laboratory Etiquette

Published: May 9, 2011

Cover Story

Laboratory Etiquette

Many lab managers still remember them from their student days—a handful of hastily stapled printouts sternly titled “Laboratory etiquette—Acceptable standards of conduct.” Those were rules to live by, and the smallest violation landed a budding laboratory scientist in front of the ticked-off chief instructor.