Overcoming resolution limits imposed by the diffraction of light has been a major breakthrough in the field of light microscopy, as evidenced by the recent 2014 Nobel Prize in Chemistry awarded to the field of "super-resolution" microscopy. With the development of super-resolution technologies there has been growing interest in the research community to correlate light-microscopy data with electron-microscopy data to further push the limits of resolution. Nikon has partnered with JEOL to address these needs.
Despite the breakthrough in resolution limit, light microscopes cannot provide the molecular level information that an electron microscope can. On the other hand, electron microscopes are traditionally restricted to fixed ("non-living") samples; a limitation that is not present in light microscopy. Light microscopes also have the advantage of enabling selective visualization of structures resulting in an extremely high level of molecular specificity. The Nikon-JEOL alliance was formed to research and develop tools that can correlate light and electron microscopy data, thereby combining the advantages that each technology provides.
"By bridging the gap between light microscopy and electron microscopy, Nikon strives to provide a true macro-to-molecular imaging solution," said Steve Ross, General Manager, Products and Marketing, Nikon Instruments Inc. "A solution that bridges Nikon's suite of light-based microscopes from stereo to confocal, multi-photon and super-resolution microscopes with JEOL's longstanding EM technology."