Image Copyright: Koponen + Hildén; Creative Commons BY 4.0
The chemical properties of atoms depend on the number of protons in their nuclei, placing them into the periodic table. However, even chemically identical atoms can have different masses—these variants are called isotopes. Although techniques to measure such mass differences exist, these have either not revealed where they are in a sample, or have required dedicated instrumentation and laborious sample preparation. Publishing in the prestigious open access journal Nature Communications, researchers at the University of Vienna report a new way for "weighing" atoms by atomic-resolution imaging of graphene, the one-atom-thick sheet of carbon.
The different elements found in nature each have their distinct isotopes. For carbon, there are 99 atoms of the lighter stable carbon isotope 12C for each 13C atom, which has one more neutron in its nucleus. Apart from this natural variation, materials can be grown from isotope-enriched chemicals. This allows scientists to study how the atoms arrange into solids, for example to improve their synthesis. Yet, most traditional techniques to measure the isotope ratio require the decomposition of the material or are limited to a resolution of hundreds of nanometers, obscuring important details.
In the new study, led by Jani Kotakoski, the University of Vienna researchers used the advanced scanning transmission electron microscope Nion UltraSTEM100 to measure isotopes in nanometer-sized areas of a graphene sample. The same energetic electrons that form an image of the graphene structure can also eject one atom at a time due to scattering at a carbon nucleus. Because of the greater mass of the 13C isotope, an electron can give a 12C atom a slightly harder kick, knocking it out more easily. How many electrons are on average required gives an estimate of the local isotope concentration. "The key to making this work was combining accurate experiments with an improved theoretical model of the process," says Toma Susi, the lead author of the study.
Publishing in Nature Communications allowed the team to fully embrace open science. In addition to releasing the peer review reports alongside the article, a comprehensive description of the methods and analyses is included. However, the researchers went one step further and uploaded their microscopy data onto the open repository figshare. Anyone with an Internet connection can thus freely access, use and cite the gigabytes of high-quality images. Susi continues: "To our knowledge, this is the first time electron microscopy data have been openly shared at this scale."
The results show that atomic-resolution electron microscopes can distinguish between different isotopes of carbon. Although the method was now demonstrated only for graphene, it can in principle be extended for other two-dimensional materials, and the researchers have a patent pending on this invention. "Modern microscopes already allow us to resolve all atomic distances in solids and to see which chemical elements compose them. Now we can add isotopes to the list," Kotakoski concludes.
Funding from the Austrian Science Fund (FWF), the Wiener Wissenschafts-, Forschungs- und Technologiefonds (WWTF), and the European Research Council (ERC) directly contributed to enabling the research.