Current approaches to immunotherapy include harnessing the power of the body’s immune system to treat tumors with cell therapies. One approach is through vaccination, a method where an antigen together with an adjuvant stimulates the body to create therapeutic T cells. Dendritic cells (DCs) are the most potent antigen-presenting cells capable of priming naïve T cells and are therefore essential tools in the activation of immune responses to tumors. They can be generated in vitro from CD14+ peripheral blood or cord blood monocytes through a maturation process. It is essential for these monocyte-derived DCs (Mo-DCs) to maintain their functional capacity of the launching antigen-specific immunity, leading to T cell activation into therapeutic T cells. Serum and undefined components found in animal- or human-derived raw materials can negatively affect this Mo-DC functional capacity. PRIME-XV Dendritic Cell Maturation CDM provides a robust method for the generation of Mo-DCs in chemically-defined culture conditions, thereby maintaining their capacity to induce T cell response.
“Our continuous research on the effects of components in media has produced convincing data that culturing cells in chemically-defined conditions improves the ability to reliably produce populations of cells that maintain desired functionality and morphology compared to culture media containing animal-derived components.” said Jessie H.-T. Ni, chief scientific officer, Irvine Scientific. “Chemically-defined media also reduces the risk of introducing foreign agents or impurities from undefined components, thereby facilitating scale-up to commercial production and the regulatory submission process.”
PRIME-XV Dendritic Cell Maturation CDM is the ideal complement to PRIME-XV T Cell CDM, the first commercially available chemically-defined medium for T cell culture. Together they provide a complete workflow free from the variability and risk of contamination that the use of animal-derived components presents. All products are manufactured using stringent raw material qualification and under Current Good Manufacturing Practices (cGMP) for consistency and reliability.