With the PureLink kits, researchers can isolate transfection-quality plasmid in about 30 minutes—up to 0.4 mg for midi scale and up to 1.5 mg for maxi scale preparation—enabling researchers to obtain high yields of low-endotoxin DNA in significantly less time and without a tedious alcohol precipitation step.
“We are committed to accelerating our customers’ journey to discovery and are excited that we can now offer customers a complete portfolio of low-endotoxin plasmid DNA isolation kits for a wide range of applications,” said Jeff Franz, general manager of sample preparation at Thermo Fisher Scientific. “The latest offering in our portfolio of plasmid DNA isolation kits offers researchers a protocol for protein expression that is simple, prompt and reliable. With a streamlined purification process, researchers can focus on higher value activities—such as vaccine development, gene therapy, and genome engineering.”
The technology behind the faster isolation time is Thermo Fisher’s innovative advanced silica membrane-based columns, utilized in combination with the Thermo Scientific FastVac vacuum manifold—enabling isolation of transfection-quality plasmid in as little as 30 minutes. Traditional anion-exchange resin-based columns rely on gravity flow to purify the DNA, and also require an isopropanol precipitation step, which takes more than two hours.
“Earlier this year, we introduced new endotoxin-free maxi, mega, and giga plasmid purification kits using the Fast Anion Exchange membrane technology,” said Franz. “Now we are adding a new advanced silica technology to our low-endotoxin portfolio in response to growing customer demand for even faster purification times. Pharmaceutical companies, in particular, require more efficient plasmid purification solutions to help fill their pipeline for new therapies as they increasingly move toward biologics.”
Low-endotoxin plasmid is ideal for transfection of a wide range of cell lines, as well as less sensitive applications such as in vivo transcription, PCR, cloning and sequencing. The PureLink Fast Low-Endotoxin Midi and Maxi Plasmid Purification Kits meet stringent industry standards for low-endotoxin plasmid DNA, with less than 1 EU/µg, and provide high-purity DNA with ultra-low levels of protein, RNA, and genomic DNA contamination, along with the productivity gains of vacuum assisted filtration.
Plasmid purified with the PureLink Fast Midi and Maxi Kits is fully compatible with and complements the workflows of the Gibco ExipCHO and Expi293 Expression Systems.
For more information about the PureLink Fast Low-Endotoxin Purification Kits or other Plasmid DNA purification and analysis solutions, visit Thermofisher.com/lowendoplasmid
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. is the world leader in serving science, with revenues of more than $20 billion and approximately 65,000 employees globally. Our mission is to enable our customers to make the world healthier, cleaner and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics, deliver medicines to market, and increase laboratory productivity. Through our premier brands—Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services—we offer an unmatched combination of innovative technologies, purchasing convenience and comprehensive services. For more information, please visit www.thermofisher.com.
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