Electrophoresis relies on a basic process — particles moving in an electric field. Known for more than 200 years, this phenomenon still drives fundamental techniques in many labs and its long history plays a role in the widespread use of the technology. Current interest lies in making the technology, faster, more accurate and more sensitive.
9 Questions You Should Ask When Buying Electrophoresis Equipment and Gels
- How many gels per experiment can you run at once in a single electrophoresis cell?
- Can you run hand cast and precast gels with the same electrophoresis equipment?
- Can you blot in the same tank as you run the gels?
- How fast can you run a set of gels with optimal performance?
- How fast can you visualize your proteins in the gel?
- Do you need any special buffers or sample buffer to run your gel?
- Does a precast gel give you the same separation as a hand cast gel?
- How fast can you transfer proteins from your gel to a membrane?
- How efficiently can you transfer your high MW proteins from your gel to a membrane?
|Top ten factors/features considered by our readers when buying an electrophoresis system:|
|Ease of use||100%|
|Availability of supplies and accessories||99%|
|Durability of product||99%|
|Low operating costs||98%|
|Fast time to results||97%|
|Low maintenance/easy to clean||97%|
|Integrated software lets you control the instrument||97%|
|Safety and health features||94%|
|The main application for electrophoresis use in our readers’ facilities:|
|Protein Gel Electrophoresis||33%|
|Nucleic Acid Gel Electrophoresis & Blotting||14%|
|DNA & Genome Sequence Analysis||9%|
|Capillary Electrophoresis Sequencing & Fragment Analysis||8%|
|Genotyping & Genomic Profiling||4%|
|Protein Sample Preparation & Protein Purification||4%|
|The main issues with electrophoresis products being experienced by users:|
|Time to results (not quick enough)||39%|
|Inconsistency in gels||31%|
|Shelf life is too short for gels||24%|
|Dangers in handling toxic chemicals to make gels||19%|
|Buffers heating up too much||15%|
|Not enough control options in the electrophoresis system||11%|
|Electrophoresis components being used in our readers’ labs:|
|Electrophoresis gel apparatus||94%|
|Reagents: gel staining chemicals, premade gels,
gel chemicals, buffers, etc.
|General lab equipment: pH meter, pipettors,
scale, stir plates, etc.
|Digital camera/gel documentation systems||72%|
|White light/UV light box||58%|
|Respondents are using or planning to use the following gel types:|
Completed Surveys: 250
For more information on electrophoresis systems, including useful articles and a list of manufacturers, visit www.labmanager.com/electrophoresis