2013 Automated Liquid Handling Survey Results

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Automated liquid handling (ALH) systems span the range from semi automated multichannel pipettors to room-sized systems. The industry is trending toward versatile, modular ALH systems—seemingly for every budget. Likewise, instrumentation, software & methods have followed the trend toward greater user accessibility.

Top 7 Questions You Should Ask When Buying an ALH System

  1. What kind(s) of dispensing technology is used? Peristaltic pump dispensing offers low prime volumes & back-flushing; microprocessor- controlled syringes have fast output & high precision. Hybrid detection systems combine both technologies in one & can even add washing functions.
  2. Is plate handling automatable? Manual plate handling can slow productivity. Automating the process with a
    compatible microplate stacker increases throughput with walk-away operation.
  3. Can it accommodate magnetic or plastic bead-based assays? If using bead-based assays, it should be
    equipped with appropriate magnets or vacuum filtration for critical wash steps.
  4. What is the volume range, and how many different sample vessel types may be used?
  5. Ask about the software—is it integrated and user-friendly? Does it allow for pre-programmed and custom protocols?
  6. What is the flow rate spectrum? A wide flow rate spectrum allows use with sensitive cell-based assays
    to viscous liquids.
  7. What assay validation data is available for this specific liquid handler? This provides proof that the
    instrument performs as indicated.

Survey respondents are currently using the following types of liquid handlers:

Stand-alone 79%
Individual benchtop workstations 54%
Self-contained multi instrument systems 31%
Other 6%

ALH is used for the following procedures in readers’ labs:

Serial dilution 58%
Plate replication 43%
Plate reformatting 42%
PCR setup 40%
High-throughput screening 37%
Cell culture 13%
Array printing 6%
Other 44%

Top ten features/factors survey respondents look for when buying an ALH:

Maintenance / service / availability of accessories and replacement parts 99%
Accuracy and precision for a wide range of compatible fluids 98%
Safe sample handling — no cross-contamination 98%
Price 95%
Size - Small footprint 92%
Rapid Throughput 91%
Maximum operator safety 85%
Pipetting Flexibility – Pipette with 384, 96, 24, 16, 12 or 8 tips 84%
Ability to expand on instrument as requirements change 83%
Sample tracking — automated recording & sample data documentation with
a choice of bar code reader options
79%

Respondents are currently using an ALH equipped with the following:

Disposable tips 88%
Fixed, washable 46%
Sound waves that use acoustic energy to transfer liquids 6%

The following errors have occurred in readers’ labs that, as a result, reduced the accuracy of the results in automated liquid handling:

Contamination of samples 43%
Selection of the wrong pipetting technique 31%
Sequential Dispensing Inaccuracies 28%
Selection of the wrong tip type used on the ALH 25%
Dilution upon dispensing 25%
Other 19%

For more information on automated liquid handlers, including useful articles and a list of manufacturers, visit www.labmanager.com/liquid-handling

See the most recent survey results

Categories: Surveys

Published In

Is Your Message Getting Lost in the Sauce? Magazine Issue Cover
Is Your Message Getting Lost in the Sauce?

Published: May 1, 2013

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