2015 PCR Product Survey Results

Polymerase Chain Reaction (PCR) is a technology used to amplify a piece of DNA across several orders of magnitude. This technique employs thermal cycling, which consists of repeated heating and cooling of the reaction for DNA melting and enzymatic replication. PCR has found applications in a variety of fields including medical and biological research, cloning, functional genetic analysis, forensics, and disease diagnosis.

By Trevor Henderson

PCR Survey SaysTop 5 Questions You Should Ask When Buying PCR Equipment

  1. Consider the type of equipment you need, whether it be standard PCR, quantitative real-time PCR (qPCR), or digital PCR.
  2. What sample formats do you require? While most users run their reactions in a 96-well format with 0.2 mL tubes, other formats are available.
  3. Consider the ramp temperature and time. Ideally, you want a system with fast temperature ramp up/cool down time, and with low error.
  4. How many users will be using the system? Systems which can run two independent PCRs at the same time might be desirable for labs with multiple users.
  5. Consider your throughput requirements. For high throughput applications, systems that can accommodate larger plate formats, or that have integrated automation and robotics may be desirable.

Types of PCR systems used by survey respondents:

Standard PCR 80%
Multiplex PCR 30%
Real-time PCR / qPCR 71%
Digital PCR 2%
Other 1%

PCR applications as reported by survey respondents:

Gene expression 53%
DNA sequencing 40%
Cloning 36%
Pathogen detection 26%
Diagnostics 24%
SNP genotyping 19%
Site-directed mutagenesis 17%
Copy number analysis 16%
Viral quantitation 11%
Microarray/miRNA/RNAi validation 9%
Microsatellite analysis 5%
Other 8%

Nearly 54% of respondents are engaged in purchasing a new PCR system. The reasons for these purchases are as follows:

Replacement of an aging system
Addition to existing systems, increase capacity
Setting up a new lab
New application requiring different instrument

Top 10 features/factors respondents look for when purchasing a PCR system:

Consistent quality 94%
Sensitivity 75%
Price 75%
User-friendly controls/software interface 66%
Ease of use 65%
Service and support 63%
Warranty 61%
Multiplexing capability 57%
Linear dynamic range 46%
Ease of installation 43%

For more information on laboratory equipment, including useful articles and a list of manufacturers, visit www.labmanager.com/lab-products

See the most recent survey results here

Categories: Surveys

Published In

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Safer Science

Published: June 11, 2015

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