Flow cytometry is a powerful technology that allows researchers and clinicians to perform complex cellular analysis quickly and efficiently by analyzing several parameters simultaneously. The technique, whereby cells suspended in a stream of fluid are passed through an electronic detection apparatus, is used for cell counting, cell sorting, and biomarker detection, among other applications.
Top 5 Questions You Should Ask When Buying a Flow Cytometer
- What type of information can you obtain in terms of cell-based assays at the level of individual cells?
- How does the company’s technology improve your level of confidence in flow data?
- How does the learning curve of the instrument’s software compare to competitive offerings?
- Will you be able to perform assays on this instrument without extensive experience?
- Can the company provide some key differentiators regarding their system(s)?
Research applications of flow cytometry, according to survey respondents:
|GFP and RFP detection||39%|
|Cell cycle analysis||16%|
|Marine sample analysis||6%|
Most common problems users experience when using their flow cytometer:
|Low event rate||50%|
|No signal/weak signal intensity||39%|
|Two or more cell populations observed when there should be one||32%|
|High side scatter background||29%|
|High background/high percentage of positive cells||21%|
|High signal intensity||14%|
|High event rate||4%|
Factors that would help users overcome their flow cytometry challenges:
|Better technical support||
Some of the most exciting applications for flow cytometry, as reported by users:
Immunophenotyping is a technique used to identify various cell populations. Antibodies identify cells by detecting specific antigens expressed on the cell surface, in the cytoplasm or the nucleus of the cells.
For more information on flow cytometers, including useful articles and a list of manufacturers, visit www.labmanager.com/tag/flowcytometry