App Note

Protein Barcoding for Next-Generation Sequencing

The combination of protein barcodes and next-generation protein sequencing technology present an inflection point for proteomics

Conceptually similar to DNA barcodes, protein barcodes represent a wealth of opportunity for precision proteomics. DNA barcodes have been front and center to major advances in molecular biology research in recent decades with numerous applications. They are used heavily in next-generation sequencing for sample identification in multiplexed libraries, improved resolution in transcriptomics, and genotype enrichment for protein engineering. 

Protein Barcoding for Next-Generation Sequencing

Protein barcodes similarly facilitate cutting-edge opportunities with expanding applications. They allow for the precise tracking and identification of proteins in complex samples, with potential applications in drug screening, detecting biomarkers, and tracking protein interactions and functions, Also like DNA barcoding, their full potential is realized with accessible next-generation sequencing technology that enables direct sequencing and identification with single-molecule resolution.

This application note explores the design and evaluation of a synthetic protein barcode library and some of its applications. Download it now to learn more about:

  • Criteria for protein barcode design and scalability estimation 
  • Methods for generating protein barcode libraries, and relative quantitation of barcodes
  • Example applications demonstrating the use of protein barcodes for screening proteins with 300-fold enrichment

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