Immunofluorescent analysis of E18 Sprague Dawley dissociated rat cortical neuronal cells (MAP2, blue) mixed with astrocytes (GFAP, red), with nuclei (green) overlay.  Cells were labeled with chicken anti-MAP2, rat anti-GFAP, Alexa Fluor Plus 405 Goat anti-Chicken (1:250), Alexa Fluor Plus 647 Donkey anti-rat (1:500), and Sytox Green. Imaged on an EVOS M7000 Imaging system.