Next-generation sequencing (NGS) continues to revolutionize the field of genomics, and the momentum continues well past almost two decades since its introduction in 2000. Since then, technological advances in long-read sequencing, single-cell sequencing, and multi-omic read outs, such as spatial analysis, have relied on the principles of NGS. Additionally, there has been clear strides made to lower the cost of NGS that make sequencing an accessible technology to global researchers.
However, when labs explore options to scale their throughput or come upon limited samples, standard library preparation may no longer be sufficient due to considerations like cost, reliability of manual methods, or required volumetric ratios for low input samples at standard reaction volumes.
In this introductory guide to NGS library prep miniaturization, you’ll learn about:
- Different types of NGS workflows and common challenges
- Benefits to automated library prep miniaturization
- The capabilities of the 'dynamic duo’ liquid handling systems
- A growing list of verified 40+ miniaturized NGS methods