Unlock the Full Value of Your Imaging Data

Figure 1. Single timepoint of a time-lapse recording of mammary epithelial micro spheroid cultured in 3D highlighting individual mitotic events.

Data courtesy of intelligent imaging group (B. Eismann/C. Conrad at BioQuant/DKFZ Heidelberg)

Figure 2. High magnification micrograph of a sinonasal papilloma, also Schneiderian papilloma and Schneiderian polyp. H&E stain. Inset shows segmented tissue and nuclei regions trained using Aivia’s Pixel Classifier tool.

Wikimedia Commons

Complex microscopy images are worth more than a thousand words—they contain valuable information pertaining to cell morphology, function, and dynamic interactions and relationships between cells. Extracting this data with standard segmentation methods requires significant manual curation, which is time-consuming, subjective, and offers poor reproducibility. Aivia software is powered by artificial intelligence (AI) to simplify image analysis, provide robust and reproducible segmentation results, and unlock all the value contained in the data. This is achieved through image restoration, virtual staining, interactive rendering, results exploration, analysis, and more—all within a single platform. Aivia releases scientists from mundane, repetitive tasks, to focus on higher level thinking and problem solving, where discovery and innovation occurs.

Accessible data analysis for biologists

Unlike many image analysis platforms that require computer science expertise, Aivia is designed to leverage biologists’ expertise. 

A trained biomedical scientist, has expert knowledge of a cell’s appearance but not necessarily how to enter the relevant parameters into software so it can detect and analyze the cells. As a result, many image analysis platforms require significant training and practice to ensure accurate results, which can prolong projects and delay publications.

Figure 3. THY1-EGFP labelled neuron in whole mouse brain processed using the PEGASOS 2 tissue clearing method. Imaged on a Leica confocal microscope. Neurons were traced using Aivia’s 3D Neuron Analysis – FL recipe.

Hu Zhao, Texas A&M University.

As an AI-driven platform, Aivia allows scientists to perform parameter-free image segmentation, for faster, more efficient imaging projects. Aivia provides the scientist a paintbrush tool to outline cells of interest, and the software fills in all the relevant parameters based on this input to detect other cells of the same type. This approach harnesses the scientists’ expertise for segmentation, and dramatically reduces time spent inputting and finetuning parameters. 

Robust, reproducible results

Human beings—especially scientists—are good at creative thinking, integrating knowledge across multiple domains, and solving problems as opposed to performing mundane and repetitive tasks. Image processing is one such task where even the most diligent individual can become demotivated and begin to make mistakes, which reduces data quality and reproducibility. Performing these tasks manually also increases the risk of bias.

Fortunately, image processing is amenable to automation. Aivia’s unique blend of AI and smart heuristics significantly reduce the challenges of underor over-segmentation and produce robust, unbiased, and reproducible results. When skilled laboratory personnel are alleviated of this tedious and timeconsuming task, they are able to spend time focusing on more complex tasks, innovation, and discovery.

Figure 4. Images of microtubules and nuclear pore complexes processed in Aivia with a 3D RCAN deep learning model to improve the image resolution.

Christian Combs, NIH; and Hari Shroff, NIBIB.

Maximizing data

Images contain a plethora of valuable data. The challenge lies in accessing it. This can be achieved by incorporating multiple imaging and analysis systems into a workflow. However, this approach requires proper training, is time-consuming and inefficient. Aivia enables powerful and fast 2-5D visualization and analysis, includes 22 applications, and 20 pre-trained deep learning models for image segmentation, restoration, and virtual staining. 

This powerful platform allows scientists to explore previously intractable enormous datasets with ease, and works seamlessly with all microscopy imaging systems and over 45 microscopy file formats. Aivia works on a local laboratory computer, as well as in a web browser, enabling your team to work with important files wherever and whenever they need. 

The Aivia platform makes AI-powered data analysis accessible for all, generates robust and reproducible data, and enables scientists to unlock the full potential of imaging data. The future of AI microscopy is here. 

To learn more about Aivia, visit:
https://www.aivia-software.com/leica-aivia