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Whitepaper

Advancements in LED Tech Transform Fluorescence Microscopy 

Recent technological leaps improve sustainability, uniformity, and control in imaging at a reduced cost

Traditional illumination sources for fluorescence microscopy present marked challenges for labs. These include the short lifespan of mercury and metal halide lamps, their substantial heat output affecting specimen integrity, high operational costs, and the environmental concerns associated with hazardous materials. Looming bans on mercury add urgency to the debates surrounding alternative lighting options. This resource delves into the scientific and practical advantages of employing advanced illumination technologies, specifically focusing on the benefits and applications of new LED-based lighting systems in overcoming these obstacles.

Fluorescence microscopy light source challenges

Traditional lighting solutions in fluorescence microscopy present multiple challenges that can hinder high-quality image acquisition along with the higher operational costs and environmental impact. Long boot up times, high energy consumption, and long wait times between power cycles affect productivity and efficiency in the lab. Further, the inability to precisely control the intensity and duration of illumination can lead to photobleaching and phototoxic effects on specimens, compromising experimental outcomes.

Early LED lighting systems were limited in their suitability for most fluorescence microscopy applications due to constraints in wavelength selection and irradiance. These technical limitations led many to dismiss the potential of LED solutions, though rapid advances in recent years have had an enormous impact on the relevance and application range of new LED technology. 

Modern solutions and their scientific value

Modern LED illumination technology offers a broad spectrum of wavelengths for greater imaging flexibility, reduced heat generation, and precise control over intensity and timing, minimizing photodamage and extending the viability of sensitive samples. This transition opens new avenues for research, including high-speed live cell imaging and dynamic fluorescence applications like Förster resonance energy transfer for capturing protein-protein interactions and precise 340/380 nm Fura-2 calcium imaging. The technology also supports emerging techniques such as optogenetics, where light is used to control cells within living tissue.

Significant reductions in energy consumption and increased longevity contribute to financial and environmental benefits. Operational improvements extend to improved workflow efficiency and mitigation of health and safety hazards.

Download this whitepaper to discover why labs are increasingly turning to advanced LED lighting systems and, in particular:

  • A detailed cost-of-ownership comparison between LED, mercury, and metal halide based lighting options currently on the market
  • How to assess illumination intensity/power for comparative measurements between lighting systems and manufacturers
  • How to improve consistency and repeatability of imaging experiments
  • How to improve specimen longevity and data quality
  • Sustainability considerations for microscopy illumination, and what to look for in new systems.
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