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Molecular Biology

Setting New Standards for Precision in Cell and Gene Therapy

ddPCR Resource Guide

Traditional, quantitative PCR (qPCR) has been an essential tool in clinical viral diagnostics but is also accompanied by its fair share of limitations. This is especially telling when dealing with lower abundance targets or template copy numbers where qPCR results often vary alongside the use of a standard curve for quantification and a subjective signal threshold. 

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This set the stage for the development of digital PCR (dPCR) in the late 90s and transformed the technique from its analog origins to a digital platform. dPCR offered absolute and variation-less quantification of nucleic acids, but unfortunately, was also limited by its unsuitability for large amplicons, low extraction throughput, and a high risk for sample contamination.

  • Quality control solutions Bio-Rad has to offer for cell and gene therapy
  • How to transition your assay from qPCR to ddPCR 
  • Viral genome quantification for recombinant adeno-associated virus (rAAV) development

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