The emergence of fluorescence- and luminescence-based assays has significantly reduced the need for radioisotope labeling in the life sciences. Nonetheless, radiolabeling remains a useful technique for several life sciences applications. 32P, for instance, is still commonly used in northern and southern blotting assays, and 125I and 35S remain widely employed in protein analysis. It is therefore crucial for lab personnel to know how to handle radioactive materials and processes within the confines of their biosafety cabinet.
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