Plants Use a Molecular Clock to Predict When They’ll Be Infected

Infected Leaves Four-week-old Arabidopsis thaliana leaves 64 hours after being infected with 5 ul of Botrytis Cinerea spore suspension.Image credit: University of WarwickPlants are able to predict when infections are more likely to occur and regulate their immune response accordingly, new research has found.

Led by the University of Warwick, the researchers discovered that a plants’ molecular clock is connected to their immune system to increase levels of resistance to infection at dawn–the time at which fungal infections appear most likely to occur, with plants unable to maintain the highest level of resistance at all times of day.

The researchers identified a single protein, JAZ6, in the plant cell which drives this time-of-day difference in the effectiveness of the immune response, with it connecting the plant clock to the immune system.

Arguing that they can now use JAZ6 to pick out the key parts of the plant immune response controlling resistance to fungal pathogens, the researchers say they can now focus on how to improve disease resistance in crops by molecular breeding.

Whilst previous research had shown that resistance against bacterial pathogens varied at different times of the day the new research has for the first time shown that the same is true for resistance against a fungal pathogen. The University of Warwick’s new research is also the first to identify a mechanism of how the internal plant clock is driving the difference in plant immunity at dawn and night.

Molecular (circadian) clocks drive the daily rhythms in many organisms, from humans to insects to plants and even marine algae. They are the internal time keepers that enable organisms to anticipate predictable changes during a 24-hour period.

“Plants are able to predict when pathogen infection is more likely to occur and regulate their immune response to combat this, with plants being more resistant to infection after inoculation at dawn compared to inoculation at night," explains lead researcher Dr Katherine Denby, of the University of Warwick’s School of Life Science. "The difference in a plant’s resistance to infection at different times of the day is driven by its circadian clock rather than daily light/dark changes, with the differences existing regardless of whether you put the plants in constant light for a day and then infect at what would be dawn or night.”

The researchers conducted the research by infecting plants with Botrytis cinerea spores every three hours over a 24-hour day and measuring the subsequent lesions that developed. They observed that the plants inoculated in the morning developed much smaller lesions and were more resistant to disease compared to those plants which were inoculated at night. Those inoculated at night had significantly larger lesions and far more growth of the pathogen in the leaves.

“We infected plants with a dysfunctional circadian clock in the morning and at night with our fungal pathogen and observed that the plant no longer had a difference in resistance at the two times of day," University of Warwick PhD student Claire Stoker says. "This pattern showed us that resistance must be driven by the plant’s internal clock.”

The research, "Jasmonate signalling drives time-of-day differences in susceptibility of Arabidopsis to the fungal pathogen Botrytis cinerea," is published by, The Plant Journal.