Lab Manager | Run Your Lab Like a Business

News

Why Fecal Transplantation Is No Joke

“There is no doubt that poo can save lives,” says researcher

Register for free to listen to this article
Listen with Speechify
0:00
5:00

toiletsFecal transplant research is just getting started, researcher says.Photo credit: iStock

These days fecal transplantation is no joke.

Fecal transplants are increasingly being used as the treatment of last resort for certain infections in the human gut and have had remarkable success treating the nursing home and hospital-acquired scourge, Clostridium difficile colitis, an infectious diarrhea that often follows antibiotic treatment. There is also preliminary evidence that the transplantation of stool from healthy individuals can be effective in treating multiple sclerosis and Crohn’s disease.

Get training in Biosafety and Biosecurity and earn CEUs.One of over 25 IACET-accredited courses in the Academy.
Biosafety and Biosecurity Course

Seth Bordenstein Seth Bordenstein Photo credit: Vanderbilt UniversityAt the same time, there has been a major increase in animal experiments involving fecal material. In one study, for example, researchers found that fecal transplants from lean mice turned sterile mice into lean mice, while fecal transplants from fat mice turned sterile mice into fat mice.

“This research is just getting started. It is driven by the new paradigm of the microbiome which recognizes that every plant and animal species harbors a collection of microbes that have significant and previously unrecognized effects on host health, evolution and behavior,” said Seth Bordenstein, associate professor of biological sciences and pathology, microbiology, and immunology at Vanderbilt University.

In an article titled “Fecal Transplants: What is Being Transferred” just published in the journal PLOS Biology, Bordenstein reviews the growing scientific literature on the subject.

“There is no doubt that poo can save lives,” said Bordenstein. Take the case of the use of fecal transplants to treat Clostridium difficile infections. According to the literature, it has a 95 percent cure rate. “Right now fecal transplants are used as the treatment of last resort, but their effectiveness raises an important question: When will doctors start prescribing them, or some derivative, first?” Bordenstein asked.

Related Article: Treating C. difficile Infection with Oral Administration of Frozen Encapsulated Fecal Material

It turns out fecal transplantation has a long history in Chinese medicine. An early-recorded application in humans was in the 4th century by a Chinese medical doctor named Ge Hong. In the 16th century, it was popular enough to get the nickname “yellow soup.”

increase in scientific studies of fecal material The dramatic increase in scientific studies of fecal material and the microbial components in feces in numbers per gram.Image credit: Seth Bordenstein / Vanderbilt UniversityInterest among Western scientific circles was minimal until the year 2010, with fewer than ten articles on the subject appearing per year in PubMed, an index of biomedical literature. Starting in 2011, however, the number began an exponential rise with more than 200 papers on fecal transplantation appearing in 2015, a trend that shows no sign of slacking.

So far most of the research has been focused on the role of the bacteria in donor’s stool. Bacteria are the most abundant active agent in the material. However, they are not the only functional player in feces, Bordenstein cautioned. “Feces is a complex material that contains a variety of biological and chemical entities that may be causing or assisting the effects of these transplants.”

Related Article: Microbiota Affect the Rate of Transplant Acceptance and Rejection

Healthy human stool contains on average 100 billion bacteria per gram. But it also contains 100 million viruses and archaea per gram. (Archaea are an understudied domain of single-celled organisms that were classed as bacteria until the 1970’s). In addition, there are about ten million colonocytes (human epithelial cells that help protect the colon) and a million yeasts and other single-celled fungi per gram.

fecal matter transplantImage credit: Jeremy Teaford / Vanderbilt

According to Bordenstein, focus on the bacterial component makes sense in some cases, such as the treatment of Clostridium difficile colitis, but in other cases, such as the possible treatment of multiple sclerosis, it is quite possible that the effects of fecal transplants may be influenced by, or possibly even caused by, their non-bacterial constituents. As a result, he calls for increased research designed to separate out the effects and interactions of each of these components.

Related Article: Immune System Promotes Digestive Health by Fostering Community of “Good” Gut Bacteria

“When scientists identify the specific cocktails that produce the positive outcomes, then they can synthesize or grow them and put them in a pill. That will go a long way to reducing the ‘icky factor’ that could slow public acceptance of this new form of treatment,” said Bordenstein.

This work was supported by National Science Foundation grants DEB 1046149 and IOS 1456778.