AMSBIO offers a comprehensive range of high quality Heparan Sulfate (HS) antibodies from F69-3G10, F58-10E4 and JM403 clones, which have been proven ideal for targeted binding of HS in Heparan Sulfate Proteoglycans (HSPG) research.

Heparan Sulfate is synthesized as the glycosaminoglycan (GAG) component of Heparan Sulfate Proteoglycans. It is expressed on the cell surface of virtually all cell types and basement membranes in mammals. It displays specific interactions with many biologically active proteins and, thus, is involved in many important biological processes.

3G10 antibody staining (green) for Heparan Sulfate on HUES7 cells 3G10 antibody staining (green) for Heparan Sulfate on HUES7 cells following Heparinase III digestion (image courtesy of the Merry Lab, University of Nottingham UK. (DAPI blue and Phalloidin purple).Credit: Merry Lab, University of Nottingham UK. Many researchers are interested in examining the dynamic distribution of HSPG in tissues and also analyzing HS structures to elucidate the biological functions of HSPG. The non-immunogenic character of HS makes this type of antibody difficult to raise, so the few hybridoma-derived mouse anti-HS antibodies such as JM403, 10E4, and 3G10 are valuable tools for HS research. Using multiple HS antibodies that recognize subtle differences in HS patterning can be useful in applications such as flow cytometry allowing multiple cell types to be directly compared.

To assist researchers, AMSBIO has produced an 'Applications Guide for Heparan Sulfate Antibodies' that brings together a wide range of information including protocols, results, and citations from customers working in this area. In addition, the guide provides an informative introduction to applications of JM403, 10E4, and 3G10 Heparan Sulfate antibodies, including use of the 10E4 and JM-403 clones together in ELISA to detect or measure HS.

To download the  Applications Guide please visit http://www.amsbio.com/heparan-sulfate-antibodies.aspx or contact AMSBIO on +44-1235-828200 / +1-617-945-5033 / info@amsbio.com.

Founded in 1987, AMS Biotechnology (AMSBIO) is recognized today as a leading transatlantic company contributing to the acceleration of discovery through the provision of cutting-edge life science technology, products, and services for research and development in the medical, nutrition, cosmetics, and energy industries. AMSBIO has in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion, and proliferation. This expertise in cell culture and the ECM allows AMSBIO to partner with clients in tailoring cell systems to enhance organoid and spheroid screening outcomes using a variety of 3D culture systems, including organ-on-a-chip microfluidics. 

For drug discovery research, AMSBIO offers assays, recombinant proteins, and cell lines. Drawing upon a huge and comprehensive biorepository, AMSBIO is widely recognized as a leading provider of high-quality tissue specimens (including custom procurement) from both human and animal tissues. The company provides unique clinical grade products for stem cell and cell therapy applications these include high quality solutions for viral delivery (lentivirus, adenovirus, and adeno-associated virus) in addition to GMP cryopreservation technology.