Lab Manager | Run Your Lab Like a Business

News

Researchers Extract DNA from Resin-Embedded Insects

An international team has now detected DNA from ambrosia beetles that were trapped in recent tree resin for less than seven years

University of Bonn
Resin samples from Hymenaea trees in Madagascar with embedded ambrosia beetles.
Georg Oleschinski / Universität Bonn

The phenomenon of using DNA from old fossils preserved in amber already inspired Hollywood—in the film Jurassic Park, scientists reproduce the DNA of dinosaurs extracted from a fossil mosquito embedded in a piece of amber and thereby resurrect them. In reality, however, the undertaking is much more difficult: all previous studies in which researchers took DNA samples from insects enclosed in tree resin were the results of modern environmental contamination and, in addition, were unreproducible, subsequently useless under the scientific method. An international team led by researchers at the University of Bonn has now detected DNA from ambrosia beetles that were trapped in recent tree resin for less than seven years. The study was published in the journal PLOS ONE.

Using so-called ancient DNA, scientists can draw conclusions about long gone times and the organisms living there. The use of organisms trapped in amber (fossil tree resin) with this finality was thought not to be possible after relatively recent fails in looking for DNA in a few thousand-year-old samples.

Resin samples with embedded ambrosia beetles.
Xavier Delclòs / Universitat de Barcelona

"Our new results show that it is indeed possible to genetically study organisms that were embedded in resin, although we do not know the time limit yet," emphasizes study leader Dr. David Peris of the Institute for Geosciences and Meteorology at the University of Bonn. The ultimate aim of the researchers is to dissolve, step by step, fundamental aspects of the DNA preservation in the resin and to determine the real amount of time that DNA in resins remains preserved.

Focus on relatively young resin samples

"Instead of looking for DNA in amber of 100 million years old or more, to dream about the resurrection of dinosaurs, we should start by detecting it in insects trapped a few years ago in resin," highlights David Peris. The resin samples used were six and two years old and came from Madagascar. To detect the DNA, the scientists established a method based on the technique called polymerase chain reaction, which makes it possible to multiply genetic material in a test tube. This method is well-known in criminology and recently became famous, as the basic technology for the detection of the SARS-CoV-2. "This method allowed us to perform several authenticity checks, so that we could say certain that the detected DNA in our experiments was indeed from the beetles preserved in the resin," explains Kathrin Janssen of the Institute of Medical Microbiology, Immunology and Parasitology, also part of University of Bonn, the second lead author.

The researchers found that water is stored in the embedded samples longer than previously thought, which has a negative effect on the stability of the DNA. In the future, the scientists plan to gradually analyze older samples with more sensitive "next generation sequencing" methods. "Investigating the time limit of DNA conservation and many other related issues is the aim of future experiments," summarizes Kathrin Janssen.

- This press release was originally published on the University of Bonn website