HPLC systems are forced to their pressure limits under fast HPLC and high resolution HPLC conditions. System back pressure issues can arise from efforts to increase efficiency and resolution such as increasing flow rates and column lengths, as well as reducing particle size.

The detection of pathogenic or spoiling microorganisms has always been an important topic in the food and beverage industry.

Excessive background from endogenous sample matrix components has always been of great concern in bioanalysis, and has become paramount today with the need for decreasing analytical run times.