The KD Scientific Legato® 100 syringe pump is used in the new Drop-Seq technology developed to allow biologists to investigate large numbers of individual cells simultaneously using RNA-seq.
The Drop-Seq1 method isolates thousands of cells with sets of uniquely DNA-barcoded primer beads in tiny nanodroplets. Three Legato 100 syringe pumps provide the three required independent flow streams of cells, micro particle buffer and oil through a specifically designed microfluidic device. The Drop-Seq method allows standard biology labs a uniquely low-cost, high yield, and high purity method for investigating and sequencing complex tissues.
For more information and protocols concerning Drop-seq, please visit www.mccarrolllab.com/dropseq.
The Legato 100 single syringe infusion pump has a flow rate range of 1.26 pl/min to 88 ml/min (syringe size dependent) with 30 lb. (13.6 kg) of adjustable force across the entire flow range. This innovative syringe pump can hold syringe sizes 0.5 µl to 60 ml.
Legato® 100 Syringe Pump Features:
- Economical single syringe infusion pump
- Holds syringe sizes from 0.5 µl to 60 ml
- High resolution, chemically resistant, color touchscreen display
- Flow rates down to the pl/min (syringe size dependent)
- Built in syringe selection table
- USB, Footswitch & TTL Interfaces for external control
- Rugged construction with cold rolled steel chassis and integrated spill dam
For more information about KD Scientific syringe pumps contact:
84 October Hill Road
Holliston, Massachusetts 01746 USA
Phone: (508) 429-6809
KD Scientific syringe pumps are for laboratory use only. They have not been approved by the FDA for human clinical use.
1Macosko, Evan Z. et al. “Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets.” Cell, Volume 161, Issue 5, 21 May 2015, Pages 1202-1214. https://doi.org/10.1016/j.cell.2015.05.002