The 2010 Geno/Grinder® offers analysts a versatile high-throughput tissue homogenizer for plant or animal tissue homogenization. The patented design with a true linear grinding motion provides the most efficient bead milling mechanism for tissue homogenization and cell lysis for extraction of DNA, RNA, proteins or enzymes. The 2010 Geno/Grinder® offers analysts a versatile high-throughput tissue homogenizer for plant or animal tissue homogenization. The patented design with a true linear grinding motion provides the most efficient bead milling mechanism for tissue homogenization and cell lysis for extraction of DNA, RNA, proteins or enzymes. The powerful and compact design enables complete disruption with or without buffer in about 1-3 minutes with high yields. The 2010 Geno/Grinder® has also been successfully used for pesticide residue extraction from fruit and vegetables as well as other organic contaminant extractions from food samples using the QuEChERS technique.
Features and Benefits
• Handles two standard deep-well titer plates or multiple vials from 0.6 to 50 mL
• Optional large clamp assembly (2199) enables processing of up to four deep-well titer plates or sixteen 50 mL vials simultaneously
• Controls allow the operator to set the run time in 5 second increments and precise vertical grinding speed between 500 and 1750 strokes per minute
• Safety interlocks and pneumatic cylinder stabilize the lid for safe operation
• Supplied with height-adjustment spacers to handle titer plates, racks, vials, and other configurations • LCD screen displays the full timer setting along with the time, in minutes and seconds, remaining in the grinding cycle
• Typical Samples include Plant materials such as seeds, stems, roots, leaves, fruits, vegetables and animal tissue.
The bead milling that the Geno/Grinder® employs is the preferred method to disrupt a variety of microorganisms and plant or animal tissues. In bead milling, grinding media such as steel or ceramic balls or glass beads are vigorously agitated inside a sealed vial or titer plate with the sample. The most commonly used grinding balls are steel ball bearings of 4mm in diameter. Disruption or cell lysis occurs as a result of the crushing action of the grinding media as they collide with the sample. Low shearing of nucleic acids can be achieved by varying the agitation speed of the mill. It is considered the method of choice for disruption for yeast and fungi and tough-to-disrupt cells like bacteria and micro algae.
The bead milling method is one of the few that totally avoids possible cross-contamination between samples because both vials and grinding media are disposable. Speed and effectiveness of disruption can be increased dramatically by increasing the density, form and amount of grinding media in the sample vial or titer plate. Tough tissues may require precooling to embrittle the sample and this also serves to preserve any temperature sensitive components such as RNA or Proteins.
SPEX SamplePrep offers a full range of cryogenic, Kryo-Tech® accessories for this purpose. We offer a comprehensive handbook of sample preparation with details of different extraction techniques and appropriate grinding media and protocols for different sample types.
For more information contact us at firstname.lastname@example.org or call 855-GET-SPEX.
Like this article? Click here to subscribe to free newsletters from Lab Manager