PCR Equipment Survey Results

Learn which applications are most common and more from our latest PCR survey results.

By Jason Kerkhof

Polymerase chain reaction (PCR) is a technology used to amplify a piece of DNA across several orders of magnitude. This technique employs thermal cycling, which consists of repeated heating and cooling of the reaction for DNA melting and enzymatic replication. PCR has found applications in a variety of fields including medical and biological research, cloning, functional genetic analysis, forensics, and disease diagnosis.

Top 5 Questions You Should Ask When Buying PCR Equipment

  1. Consider the type of equipment you need, whether it be standard PCR, quantitative real-time PCR (qPCR), or digital PCR.
  2. What sample formats do you require? While most users run their reactions in a 96-well format with 0.2 mL tubes, other formats are available.
  3. Consider the ramp temperature and time. Ideally, you want a system with fast temperature ramp up/cool down time, and with low error.
  4. How many users will be using the system? Systems that can run two independent PCRs at the same time might be desirable for labs with multiple users.
  5. Consider your throughput requirements. For high-throughput applications, systems that can accommodate larger plate formats, or that have integrated automation and robotics may be desirable.

Types of PCR performed by survey respondents:

Standard PCR 77%
Real-time PCR / qPCR 76%
Multiplex PCR 34%
Digital PCR 2%
Other 8%

PCR applications as reported by survey respondents:

Gene expression 58%
DNA sequencing 42%
Pathogen detection 32%
Diagnostics 31%
Cloning 31%
SNP genotyping 27%
Copy number analysis 19%
Viral quantitation 18%
Microarray/miRNA/RNAi validation 18%
Site-directed mutagenesis 15%
Microsatellite analysis 12%
Other 7%

Nearly 50% of respondents are engaged in purchasing a new PCR system. The reasons for these purchases are as follows:

Replacement of an aging system
Addition to existing systems, increase capacity
New application requiring different instrument
Setting up a new lab

Top 10 features/factors respondents look for when purchasing a PCR system:

Consistent quality 93%
Sensitivity 75%
User-friendly controls/software interface 71%
Price 70%
Ease of use 70%
Service and support 61%
Warranty 54%
Multiplexing capability 44%
Linear dynamic range 41%
High-throughput ability 37%

For more information on PCR equipment, including useful articles and a list of manufacturers, visit www.labmanager.com/PCR

Categories: Surveys

Published In

Raising Safety Awareness Magazine Issue Cover
Raising Safety Awareness

Published: June 11, 2018

Cover Story

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