Ultraviolet-visible (UV-Vis) spectrophotometry is arguably the most common as well as one of the oldest forms of absorption-based analysis. UV and visible regions of the electromagnetic spectrum are contiguous: UV wavelengths range from 10 to 4000 angstroms; visible wavelengths range from 4000 to 7000 angstroms.
Top 5 Questions You Should Ask When Buying a UV-Vis Spectrophotometer
- For what applications will you be using the instrument for? This will help you determine the detection range you require. Don’t forget to consider future applications that may require a broader range
- What range of stray light performance are you comfortable with for your application and budget?
- Consider what sort of samples you’ll be working with in order to determine what absorbance range you will need in your UV-Vis spectrophotometer. For example, if it is a turbid or concentrated liquid or a solid sample that is optically thick, you may require a working absorbance range between 5 Å and 8 Å or higher.
- What level of throughput and reliability do you need?
- How much will the instrument cost? Don’t forget to factor in the cost of maintenance, etc., along with the cost of acquisition.
Types of UV-Vis spectrophotometers used by survey respondents:
Top 10 UV-Vis spectrophotometer uses according to survey respondents:
|Biochemistry and biology||22%|
|Cell culture analysis||4%|
|Food safety and testing analysis||4%|
|Color / dyes||3%|
Nearly 55% of respondents are engaged in purchasing a new UV-Vis spectrophotometer. The reasons for these purchases are as follows:
|Replacement of an older spectrophotometer|
|Addition to existing systems|
|Need an instrument that has a broad range of accessories|
|Need an instrument that is simple to operate and maintain|
|Need an instrument that provides faster acquisition and analysis of data|
|Need an instrument that provides excellent reproducibility|
Top 10 features/factors respondents look for when purchasing a UV-Vis spectrophotometer:
|Better Sensitivity and Resolution||74%|
|Ease of Maintenance/Low Operating Costs||68%|
|Service/Support in General||49%|
|Faster Acquisition and Analysis of Data||36%|