Webinars: Application of RNAscope for Spatio-temporal Visualization of RNA Species in Plant Tissue Samples

Application of RNAscope for Spatio-temporal Visualization of RNA Species in Plant Tissue Samples

Join Lab Manager and our experts as we discuss how this modified protocol on the plant leaf tissues can be adapted easily on other plant species and provides a powerful tool of RNAscope for RNA visualization

Friday, October 23, 2020
11 AM EDT

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RNAscope multiplex fluorescent RNA-ISH detection is a powerful and sensitive tool for spatio-temporal RNA species detection in tissues. However, due to plant tissues' morphological and structural characteristics, its implication was not reported until Solanki et al., 2020 (https://doi.org/10.1186/s13007-020-00614-4) presented a modified protocol of tissue preparation and probe hybridization. This modified protocol on the barley leaf tissues can be adapted easily on other plant species and provides a powerful tool for plant researchers and cell biologists. In this webinar, we will discuss a practical approach of carrying out RNAscope assay using a parallel comparison of plant and animal samples to avoid common pitfalls and increase the chances of a successful experiment.

Learning objectives:

  • Tissue preparation
  • Tissue pretreatments
  • RNAscope V2 assay
  • Sample visualization and image analysis

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