Live Air Date: Wednesday, June 11, 2014
Peptide mapping is probably the most powerful technique for detecting low levels of post translational modifications of proteins, especially for ones that are large and complex. The key to successful peptide mapping is generating reproducible, high resolution separations of peptides so that any subtle differences between sample components can be visualized. In this webinar, sample preparation techniques and LC instrumentation parameters will also be discussed in an effort to improve reliability. Additionally, the influence of using Aeris Core-Shell column technology for improving peptide mapping will be introduced.
As an attendee, you will learn more about:
- Strategies for developing peptide map applications that solve protein characterization challenges
- Sample preparation tips for improving reproducibility of peptide maps
- Optimizing UHPLC capabilities to improve the resolution of peptide separations
- Understanding how Aeris Core-Shell columns deliver higher resolution peptide separations
Michael McGinley is a senior product manager at Phenomenex where he is responsible for technical aspects of Phenomenex’s HPLC and bioseparation product portfolios. Prior to joining Phenomenex in 2002, Michael was at Amgen where he held several positions over a dozen year span, including associate scientist, project manager, and laboratory head. Michael received his degree in Biochemistry from the University of California at Berkeley and his expertise includes HPLC method development, proteomics, biofuel analysis, oligonucleotide chemistry, protein characterization, and protein purification with over a hundred publications in the respective fields.