Fast and Simple Free Fatty Acids Analysis Using UPC2/MS

The UPC²/MS FFA analysis described here provides a simple and fast method with a significant reduction in analysis time compared to alternative techniques such as GC/MS, which requires FAME derivatization.

by Waters CorporationJul 14, 2014|1 min read

The UPC²/MS FFA analysis described provides a simple and fast method with a significant reduction in analysis time compared to alternative techniques such as GC/MS, which requires FAME derivatization. In addition, the organic layer extract containing the lipids can be injected directly into the system, omitting the need for solvent exchange for compatibility with reversed-phase LC methods.

Demonstrates the separation of free fatty acid (FFA) species based on chain length and number of double bonds
No derivatization is required, which results in easier and fast sample preparation and eliminates artifact formation
Organic phase lipid extract can be directly injected onto the system, saving time and reducing cost per analysis
Less than three-minute chromatographic separation is up to 10X faster compared to GC/MS
Unlike GC/MS, low volatile and very long chain fatty acids (>24 carbon atoms) can be easily analyzed with UPC²

Get the full application note here.