Lab Manager | Run Your Lab Like a Business

White Papers and Application Notes

A man riding a bike in a bike race for which he will need to take tramadol for his acute pain
Photo provided by Thermo Fisher

A Fully Automated Workflow for Dried Spot Analysis

Rapid quantitation of tramadol and its metabolites

Thermo Fisher Scientific

Tramadol is a synthetic opioid that is a centrally-acting analgesic and is widely prescribed to relieve acute or chronic pain. Dried spot analysis, particularly DBS, has become an emerging technology in clinical research and toxicology applications such as inborn errors of metabolism, therapeutic drug monitoring research, forensic toxicology, and anti-doping. While dried spot analysis has gained popularity over the years, the traditional workflow still utilizes manual disc-punching followed by a combination of sample preparation procedures, including various extractions, evaporation, and reconstitution for downstream analysis. The whole process can take up to several hours.

This technical note demonstrates a fully automated workflow for dried spot analysis. The innovative integration of sample preparation into online analysis utilizing a revolutionary dried spot autosampler capable of flow-through desorption, followed by a two-dimensional TurboFlow UHPLC-MS/MS analysis is suited to handle sample cleanup from various biomatrices, separation, and identification.

Download this technical note now to learn more about the Transcend DSX-1 system, courtesy of Thermo Fisher Scientific..