White Papers and Application Notes

A New, Innovative Process for Clarifying and Sterile Filtering Cells for Protein Purification Workflows

A New, Innovative Process for Clarifying and Sterile Filtering Cells for Protein Purification Workflows

Explore a new efficient alternative to clarify and sterilize mammalian cell cultures

Pall Corporation

Centrifugation and filtration have been widely accepted as techniques required for clarifying complex cell cultures to recover extracellular proteins such as monoclonal antibodies (mAbs). However, these steps can be time consuming and costly for labs growing their cultures in 24-well plates.

This scientific brief offers an alternative to the use of centrifugation/filtration/flocculation to clarify and sterilize mammalian cell cultures. It describes an assessment of a new 24-well clarification and sterile filtration plate and a 24-well sterilization-only filter plate for the recovery of proteins present in the supernatant.

Access the application note, courtesy of Pall Corporation.