Carryover is a phenomenon where you see a peak from the previous injection remaining in the current injection. When basic compounds are analyzed with a typical C18 column, their peak shape can become deformed (e.g., tailing) due to nonspecific interactions with residual silanols on the surface of the packing materials.
LC/MS/MS is one of the most powerful techniques for a quantitative assay due to its superior selectivity and sensitivity. Having a high quality HPLC system to reduce carryover and ensure high throughput is essential for today’s laboratory.
Download this resource now to learn more about a future without carryover, courtesy of Shimadzu.