Enzyme-linked immunosorbent assays (ELISAs) are a type of multiwell plate-based technique used to determine the concentrations of antibodies, antigens or other proteins in a biological sample using chromogenic, fluorescent, or luminescent readouts. Since their development in the 1960s, they have become a staple in many diagnostic and biomedical research labs, demonstrating specificity, sensitivity and reproducibility across a range of applications. Common examples include the detection of various infectious diseases and pregnancy tests.
While there are many advantages to using ELISAs, they must be appropriately set-up and optimized to ensure reliable results.
In this guide, you’ll learn about:
- Choosing the right assay
- Sample handling and dilution
- Proper plate washing techniques
- And more!
Download this guide for an overview of the different types of ELISA and to learn tips for ensuring the success of your ELISA experiments.