Light microscopes are diffraction limited, which means they are unable to resolve individual structures unless they are more than half the wavelength of light away from one another. With traditional widefield fluorescence microscopy, out-of-focus light from areas above or below the focal plane causes additional blurring in the captured image.
Download the complimentary app note to learn more about:
- How deconvolution reconstructs an ideal image made from a collection of smaller point sources
- Deblurring, inverse filter, and constrained iterative algorithms
- Deconvolution in practice