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How Advancing Spectrophotometric Flexibility Works

Thermo Fisher Scientific has developed the Thermo Scientific Multiskan GO UV-Vis spectrophotometer which offers free wavelength selection for both 96- and 384-well plates and various types of cuvettes.

by Thermo Fisher Scientific
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Problem: With uses in DNA, RNA and protein analysis protocols, microplate spectrophotometers are an integral component to numerous research applications. Providing a quantitative measure of light absorbance through a sample, these instruments need to be able to recognize a broad spectrum of light wavelengths. However, different assays require the use of various light wavelengths, commonly ranging from ultraviolet (UV) to near infrared (IR), to provide optimal results. The process of determining an ideal wavelength and the switching of filters between each assay can prove both problematic and time-consuming. This lack of flexibility can limit throughput and make simple protocols increasingly complex and lengthy. In addition, most researchers have a preference with regards to using microplates or cuvettes with specific sample types, and this choice can be limited by restrictions of the instrumentation.

With many laboratories focusing on increasing efficiency and consequently experimental throughput, complicated set-up and analysis steps can also be extremely time-consuming. Furthermore, with multiple users often sharing a single instrument, researchers for who English is not their first language may experience issues with understanding highly technical instructions.

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Solution: In order to circumvent these issues, laboratories need to be supplied with a highly flexible spectrophotometer, which is easily adaptable to a number of different applications. In order to meet this need, Thermo Fisher Scientific has developed the Thermo Scientific Multiskan GO UV-Vis spectrophotometer which offers free wavelength selection for both 96- and 384-well plates and various types of cuvettes. Any measurement wavelength between 200 and 1000 nm can be selected at any time with the advanced monochromator-based system. Using the spectral scanning feature, the whole spectrum of a sample can be scanned in 1 nm increments in a few seconds to identify the optimal measurement wavelength for a new assay. This not only saves valuable time, but also ensures that the most accurate results are obtained from each assay performed. The Multiskan ® GO uses

Both the microplate chamber and cuvette holder of the Multiskan GO are equipped with temperature control up to 45 °C to ensure that sample integrity is maintained during temperature critical applications such as enzyme kinetic studies or cellular assays. Shaking speed can also be selected to ensure that samples within the microplate remain homogenous.

As an energy efficient system, the Multiskan GO encompasses an advanced power save function, reducing energy consumption by over 70%. In addition, this spectrophotometer conforms to the Restriction of Hazardous Substances (RoHS) directive, which guarantees that the reader is free from harmful materials, such as heavy metals, making it extremely environmentally friendly.

The Multiskan GO provides a further level of operator flexibility with the option to be controlled as a stand-alone instrument, or via the Thermo Scientific SkanIt software. Its internal software and large color display make it convenient for quick and simple measurements. Preprogrammed settings enable the fast and convenient implementation of commonly used protocols. Alternatively, even the most complex and demanding applications can be easily set-up using the intuitive SkanIt® software for PC control. Taking into consideration that not all users will be fluent in English, both the internal and PC software are available in multiple language versions, including English, French, German, Spanish, Portuguese, Russian, Chinese and Japanese.

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