When large quantities of a protein of interest are needed, especially for characterization efforts, alterations of methodology for the cultivation and harvesting of cells may be required. Harvesting multi-liter volumes of cells is a time-consuming process and typically requires additional centrifuges to complete the workflow. Sufficient cell growth and protein expression require a robust shaker capable of shaking liter-scale cultures at high rpm and a bioreactor capable of maintaining a complex culture environment as you scale-up from initial inoculum to final culture.

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