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Mastering Chromatography Sample Prep: The Key to Accurate Analysis 

When to avoid direct injection and prepare samples thoroughly 

Proper sample preparation is a crucial aspect of liquid chromatographic (LC) analysis. It is a process that not only helps enrich target analytes but also enables sample clean-up while avoiding matrix effects. LC helps separate and detect the chemical components of substances for analytic interest. Using LC, the user can take a sample mixture and quantifiably identify its components. Paired together with mass spectrometry (MS), LC-MS helps researchers physically separate target analytes in a test sample and characterize them using mass-based detection.

The direct injection method is among the most common LC-MS sample prep protocols utilized by researchers. In direct injection, the sample, internal standard, and diluent are directly pipetted into the autosampler followed by centrifugation and analysis. While direct injection provides for a streamlined workflow, it is not the only method and has its fair share of weaknesses.

In this article, you will learn about:

  • Quantifiable separation as LC effortlessly disentangles complex mixtures, allowing you to quantify the individual components with unparalleled accuracy
  • Versatile nitrogen evaporators, tailored to match your lab's specific needs, facilitating seamless sample preparation for LC-MS
  • Unlocking the true potential of your samples, paving the way for efficient characterization and profound insights
  • Understanding when to use and avoid direct injection as a sample prep protocol for LC-MS and alternatives to consider for effective sample preparation 

Download the article now to improve your lab's LC-MS sample prep.

learn more about direct injection and its alternatives, and how these processes can improve your lab’s LC-MS sample prep

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