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App Note

Automating the Oxford Nanopore Ligation Sequencing Kit

Library preparation and sequencing beyond the walls of the traditional laboratory

Long read sequencing is particularly well suited for detection of large genomic mutations, coverage of long repeat regions that confound short read assemblies, and identification of signatures that can be lost due to PCR amplification (including relative abundance in metagenomic samples and nucleotide modifications present on original DNA). 

Automating the Oxford Nanopore Ligation Sequencing Kit

Oxford Nanopore Technologies (ONT) long read sequencing of single-stranded DNA and RNA moving through nanoscale pores has been a major technological achievement in genomic research. Its advantages include the use of a small, portable sequencer that can be deployed in the laboratory or the field, low capital cost requirements, rapid turnaround times, and a user-friendly bioinformatics pipeline that allows real-time analysis during sequencing.

MIRO CANVAS is a digital microfluidics (DMF) platform that allows low throughput workflow automation for complex protocols, such as NGS library preparation. The system is compatible with a wide range of reagents. This application note describes the results that can be expected when using the ONT Ligation Sequencing Kit in a protocol developed for the MIRO CANVAS. The resulting research use only libraries can then be sequenced using ONT sequencing platforms.

Download the app note now to learn about the following key benefits:

  • Library preparation using the 1D ONT Ligation Kit is fully automated on the MIRO CANVAS
  • 75 % reduction in reaction volumes compared to manual library preparation
  • This protocol has been demonstrated on MIRO CANVAS using 1 μg of high quality, high molecular weight input DNA
  • 2 hr 30 min run time
  • N50 comparable to manual library prep

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