Cell Line Characterization Methods Reviewed

A variety of cell line characterization methods have been useful in the detection of cross-contamination and/or misidentification of cell lines. These include karyotyping, isoenzyme analysis, DNA properties, viral susceptibility, and specific antigenicity. The work of Gartler described earlier (1) shows how, in an unusual situation, analysis of a single isoenzyme, could reveal contamination and the probable source of contamination. However, if Henrietta Lacks did not express the gene for Type A G6PD or was Caucasion rather than of African descent, a single isoenzyme analysis would be uninformative. Isoenzyme analyses have proved to be very useful in the detection of contamination involving cells of different Orders or distantly separated genera. For such situations electrophoretic mobility profiles of several isoenzymes are compared with standard profiles in order to deduce whether the cells are human, mouse, rat, dog, etc. (14). In other words, the method has usefulness for cases of interspecies contamination and very little usefulness for intraspecies contamination. Also, while isoenzyme analysis can be used to identify the mammalian order from which the cell line originated, ordinarily it cannot be used to identify the individual donor. The publications of Nelson-Rees and his associates (3,4,5 ) illustrate how severa which the cell lines belongs, l approaches, some requiring great expertise, needed to be brought to bear on identification.

Human cell line identification and authentication can be performed with sensitivity and reproducibility by DNA profile methods. For most situations, adoption of the preferred method of the forensic scientists, namely Short Tandem Repeat (STR) profiling is chosen. The method combines PCR amplification of 13 hypervariable regions of microsatellite DNA followed by DNA sequencing of the amplified DNA. Because the profile is unique to each individual, it provides the information for the identification and authentication of each cell line. The profile can be recorded as a series of numbers in a bar code, thereby facilitating its use in a database (9).

This is the method now used by major repositories for the authentication of their human holdings. The cost of DNA profiling by a commercial lab varies considerably, and may be as low as $100-200. The cost to a core facility, with purchased kits can be as low as $10-30 per DNA sample. Such costs are insignificant when compared to the damage caused by research and publications involving misidentified and cross-contaminated cell lines. It behooves the lab manager to ensure that the best science is performed under his/her watch, and this includes cell line authentication.

Laboratory Technology