Immunophenotyping with flow cytometry presents challenges that can result in compromised data accuracy and reliability. Traditional methods require preparing single-cell suspensions through enzymatic digestion or mechanical disruption, which can alter surface marker expression and lead to misinterpretation. Additionally, debris and dead cells interfere with target cell detection, necessitating meticulous sample handling.
Centrifugation exacerbates these issues by compromising sample heterogeneity and cell integrity, introducing variability, leading to cell loss, and affecting subpopulation proportions. This variability can result in mechanical stress further damaging delicate cells, producing inconsistent and unreliable data.
A new automated liquid handler using the Curiox C-FREETM technology allows centrifuge-free preparation of samples for immunophenotyping, eliminating the mechanical stress and variability of centrifugation, preserving sample integrity. This system ensures consistent processing, minimizes rare cell population loss, and enhances reproducibility. The technology not only improves data accuracy and reliability but also streamlines workflows, reduces hands-on preparation time, and frees lab personnel to focus on data analysis and other critical tasks, thus increasing overall efficiency and productivity.
A centrifuge-free approach to staining and lysis consistently retains lymphocytes, Treg cells, and memory cell subsets while achieving higher live cell leukocyte frequencies. Validated by leading laboratories, this method underscores its value in clinical diagnostics and research methodologies by reliably preserving sample integrity and enhancing immunophenotyping precision.
Download the comprehensive poster to learn:
- How automated immunophenotyping systems for flow cytometry improve sample integrity and debris removal
- Comparative analysis between centrifuge-based and centrifuge-free methods
- Practical applications and benefits in clinical and research settings
- Detailed validation results from Charles River Laboratories
- Steps to integrate this system into your workflow for enhanced efficiency
