ELISA methods have become widely used across various fields of biology, from healthcare and food safety to cancer research and vaccine development. Whether you need to measure gluten in beer or neutralizing antibodies in serum, there is an ELISA available to provide answers to your scientific questions. Despite being first introduced over 50 years ago, ELISAs continue to be an essential tool for many researchers.
In this eBook, you’ll learn about:
- The history of the ELISA
- Working with ELISAs
- ELISA applications
- Lab automation for high-throughput ELISAs
- And more
ELISA 101
ELISA EXPLAINED:
from basics to
practical application
2
Contents
What is an ELISA .....................................................................3
History of the ELISA ..........................................................3
Direct ELISA ........................................................................ 4
Indirect ELISA ..................................................................... 4
Competitive ELISA ............................................................4
Sandwich ELISA .................................................................4
Working with ELISAs .............................................................. 5
Steps to run a sandwich ELISA assay .......................... 5
Work?ow of an ELISA protocol ......................................7
How to set up an ELISA and
perform basic analysis .....................................................8
How to con?gure an ELISA
endpoint protocol .............................................................8
ELISA applications .................................................................9
Food and beverage .......................................................... 9
Gluten quanti?cation in beer ...................................9
Determine a?atoxin in cannabis ..............................9
Protein quantitation ........................................................ 10
Chemiluminescent VEGF ELISA .................................. 10
Quantitate Interleukin-8 ................................................ 10
Lab automation for high-throughput ELISAs .................. 11
2
Introduction
ELISA methods have become widely used across various ?elds of biology, from healthcare and food
safety to cancer research and vaccine development. Whether you need to measure gluten in beer
or neutralizing antibodies in serum, there is an ELISA available to provide answers to your scienti?c
questions. Despite being ?rst introduced over 50 years ago, ELISAs continue to be an essential tool
for many researchers. This e-book provides an introduction to ELISAs and demonstrates how you
can e?ectively use them in your own research.