Hello everyone, and thank you for attending Lab Managers Automation Digital Summit. My name is Mary Beth DiDonna and I'll be moderating this discussion. Welcome to this session, fully automated NGS sample preparation using a digital microfluidics platform. The Miro NGS Prep System is a compact digital microfluidics platform, which enables full walkaway automation of next generation sequencing or NGS library preparation and hybridization protocols. The system uses novel technology to automate complex protocols, greatly reduces reagent usage, and provides compatibility from multiple N G S platforms and innovative microfluidics cartridges used to automate all protocol steps, including mixing thermal cycling and bead cleanups. Integra will highlight use cases and results based on protocols for long read sequencing, such as PAC bio sequencing, as well as short read sequencing applications including whole genome sequencing and target enrichment. Please send us your questions or comments at any point during this presentation. Our speaker will address your questions during the q and a session following her presentation. To ask a question or leave a comment, simply type your query into the q and a box on the right hand side of your screen. We'll try to address as many questions as possible during our time together, but if we run out of time, I'll forward any unanswered questions to our speaker and she may be able to respond to you directly if possible. Additional resources and a certificate for this presentation are located on the right hand side of your screen in the handout section. Please be sure to answer our special audience poll question for your chance to win a gift card courtesy of Lab Manager. I'd like to remind you that the recording of this webinar will be available for free OnDemand viewing after the conclusion of this event, and I would like to extend a special thank you to our sponsors who support allows Lab Manager to keep these webinars free of charge for our readers. So with that, I'd like to introduce our speaker for this presentation. Dr. Fay Christodoulou completed her PhD degree at the European Molecular Biology Laboratory in Heidelberg, Germany. Her work on r n a Biology and Evolutionary Developmental Biology has been published in Nature Cell and Science. She was awarded first prize of the SS f e E Innovation Project for her scientific contributions and has been recognized as ninth in the list of top 25 women leaders in biotechnology by Healthcare Technology Review. Dr. Christodoulou was selected to join the Endeavor Global Network of high impact entrepreneurs, thus becoming Endeavor Greece's third female entrepreneur in 2022. She was included in Fortune Greece's list of 40 under 40. Hey, thanks for joining us today. Welcome to our webinar. I'm Fay Christodoulou, the Chief Scientific Officer in microfluidics at Integra Biosciences. I will be talking about our new product, the mural canvas that enables who fully automated next generation sequencing sample preparation using digital microfluidics. Our mission at Integra is to accelerate science together. We leverage microfluidics to help scientists reimagine lab automation, which today is very much like the public transit system. It requires a lot of people, it requires large budgets and also a critical mass of riders that are commuting from one direction to another to justify carving a route. And then once the route is planned and established, there's little flexibility in changing it. So we bring a new solution to the table where lab automation is now accessible for any sample at any moment. There is no need to any anymore batch samples, no need for a large budget, and also, uh, offer great flexibility and versatility in changing protocols and an overall user friendliness that allows anyone with minimal training to prepare a sample for next generation sequence. Neuro Canvas is a compact microfluidics platform that enables true walkaway automation of N G s sample preparation products. It utilizes a disposable electronics free cartridge where reagent volumes can be reduced, and it is also compatible with any reagents or kits that we have tried so far and can perform next generation sequencing library preparation for any sequencing, and that makes it both reagent, but also sequence the agnostic automation platform. So let's take a look at how it works. Um, neural technology uses electromechanical forces to actually droplets on an array of electrodes. And as you can see on the schematic, the droplets are always sandwiched between hydrophobic surfaces so that they can easily slide every time that we actuate an electrode on what you see on the left open array of electrodes, uh, used to route droplets in any direction. This is fully programmable and, uh, in the setup that you see here, the droplets are never indirect contact with the electrodes, uh, and they're always surrounded by air. So our matrix is air. Now, this compact system has a lot of complexity under the hood, and specifically here you're facing a mirror canvas with a lid open, and the first thing to notice is how the electrode array is actually permanently part of the instrument. Um, underneath on the right, you see underneath this open array of electrodes, which is close to 1000 individually controlled electrodes, there are heaters, uh, and coolers. They're combined. There's four locations as you see demarcated in black, and then in, uh, light blue, there's four locations of magnets that get engaged and disengaged to allow, uh, beads and suspension to pay it for a different product. So with this setup combined, of course with the electro wetting forces that allow droplet mobility, uh, on the left we have the list of, uh, unit operations that are possible using the MI Canada system. And so you can dispense reagents, you can merge, uh, different droplets, you can mix it, the reagents that, that you merge, uh, either very gently through diffusive electrowetting forces or very thoroughly, uh, just like you do when you pipe it essentially. And of course, thanks to the heaters that we have, it's possible to incubate above or below room temperature. It it is actually possible to conduct, uh, P C R thermocycling, and that is a unique offering of the miral canvas as a, the sole digital micros, uh, product out there that allows, uh, P C R cycling for as many cycles as are needed for a protocol. It is possible to engage disengage magnets or pelleting of magnetic particles, uh, washing these beads after pelleting or resus suspending and washing with different buffers. And it's possible to elute off of these beads. And, uh, in the setup that I explained earlier, uh, it is a unique possibility to interface with traditional channel microfluidics, and that's what allows us to have the thorough mix. So the consumable of the mial canvas is actually single use. It's very easy to dispose and has 12 inlets, as you can see in the image where the sample is loaded, the different reagents and also the sample is removed, recovered from, um, at the end of the arm. So all of the reagents are contained in the sealed cartridge in a way that secures against any cross-contamination concerns, uh, even when in P c r reactions amplicons are produced. So the cartridge is a strong electronic spray. It's just plastic as you see on the right breakdown of of the main cartridge components. And this simplifies a lot when we manufacture the quality control. And it also assures reliable performance. It has, uh, as part of it three reservoirs. And these are really useful to keep large volumes for, uh, reagents such as ethanol or water or wash buffers that get repeatedly used in a protocol flow. And there's also waste chamber that can contain all of these waste that gets generated throughout the, the workflow from beginning to end. So what happens when you close the lead and initiate a run, you see on the left a video of, uh, color droplets that move in different directions. Again, this is very programmable and adjustable. And on the right in the video, you will see a D n A droplet traveling down merging with the reaction master mix, and then later with magnetic beads and suspension, these two will mix around diffusely, and eventually when we engage a magnet, uh, they will form a palette from where we can elute or proceed with washes. So we designed the new canvas to make it really, uh, easy to use so that any operator minimally trained or maximally trained is capable of conducting quality, uh, uh, library preparation experience. The experience of what you see on the left that we're all familiar with a multi-page protocol that we have to underline and be really cautious, step by step to execute without errors, is really now radically simplified in just a few screen transitions on a touch screen where you're simply, uh, guided how to load the reagents and initiate the run, and then you walk away and return only to collect your product rather. We also designed the ME canvas so that it is self installable, um, and portal. So here you see an image of the mural canvas in the backpack, an impact on the right. There's another image of, uh, some of our customers that took the canvas in the field, uh, for first of its kind not only in C two sequencing experiment, but also in C two library preparation, followed by C two sequencing for field applications. And in this particular example, the mural canvas was actually powered by, uh, solar fed batteries, which is, uh, uh, really exciting, uh, event for us. So what protocols are offered right now, uh, automated in mural canvas, there is a list and we have demonstrated the reliable automation of five complex protocols so far. And this list will keep growing. The, there are whole genome sequencing applications, such aspac Bio Smart Build prep, uh, keep version three, and that is for long read sequencing. We have the nano for genomic d n a violation for long read sequencing, also a modified version of that protocol in the UROC canvas, which allows ultra long genomic d n a violation. Then for short read, we have the Illumina D N a PCR free prep fit for rapid whole genome sequencing and the for whole exome sequencing applications, the twist, human core exome, fast hybridization target enrichment. And so as you can see, both whole exome and whole genome sequencing applications are served at the moment. Uh, we will be adding in the future also RNA-seq applications, and this menu will expand further. But so far with the list that you see, uh, we have actually sequenced product libraries in four different sequencing technologies, including Illumina, including, uh, element PacBio. And so for all of these protocols we saw in the list, uh, the hands-on time is really limited to the timestamp loading the MI canvas with the reagents and the sample. And from there, automation is truly walk away. So as I mentioned, you come back either two hours later or five and a half hours later to simply collect a product library and proceed with sequence. So the digital microfluidics technology, in fact, has an added value here that it also allows to reduce the reagent volumes for every protocol. In some cases, the volume redactions go all the way up to 75% achieving significant cost savings for, for the kid. That is huge. So the customers we have so far sold the MI to are using it for diverse, uh, list of applications that are categorized in three main pillars as you see here. And the first is of course, in long read sequencing. This is a really wide use where the digital microfluidics, um, gentle forces are ideal for the high molecular weight d n a, and they ensure that the quality of, of, uh, the, and the integrity of this d n a long molecule is maintained throughout the, throughout the protocol step, uh, to ensure long read sequencing results. Then, uh, in the middle, we have automation of very tedious and long, uh, protocols that actually, uh, are very error prone sometimes because of the multiple steps and also require a lot of skill. Uh, here, hybridization and capture is, is, uh, the, the twist protocol, for example. Uh, and what is special and, and interesting about this protocol is that it actually allows a higher throughput to be accommodated by the MI canvas where multiple libraries can be combined in a pool and then enriched and captured together as, uh, using just one cartridge of the MI canvas. So this is one way to multiplex using the instrument. And last but not least, in clinical applications of sequencing where actually the fast, uh, turnaround time of library preparation is of essence, um, because waiting for batching, uh, or staffing cannot be afforded, the MI canvas is an excellent feat, uh, to serve that customer. Now, you know how the product works. Let's take a look at some data, uh, generated using the mural canvas. So here, all of the data presented in the next slides, uh, remember they're coming from a hundred percent automated runs, uh, walkaway, and the duration of the runs, as I mentioned earlier, can vary between two hours to five and a half hours for the protocols we we're offering. This is really a matter of the steps involved and the incubation's required, and we conduct the automation approximately the same time as it's done manually. So mural canvas has been used for whole genome sequencing, libraries for long grade sequencing for both, as I mentioned, uh, Nanopore and PAC Bio. And we'll start with PAC Bio. And here you see a representative redistribution profile from a mural canvas, uh, generated library. We used in this particular, uh, example, the, the latest fit by pack by the SMART BE Prep, uh, version three. And this specific fit was designed to allow lower D n a input as well as, um, consolidation of of the size selection, uh, process, which is now part of the full automation workflow. So end-to-end, this experiment, uh, can be automated using the MI canvas. And for these results, we use one microgram input of N A 2 4 3 8 5 A choal standard. Um, on the right, you see the, the key sequencing run quality metrics such as the number of the reads, the hi-fi yield, uh, the mean read length and, uh, and quality. And all of these numbers are at the expected levels. And then the same exact number of structural variants, also the same type of structural variants and same distributions were reported in the mural canvas library compared to a representative manual library. As you see here on the right, uh, table and also in the bar chart umbrella, one of our customers, the Broad Genomics platform, has actually used the canvas to, to prepare several HiFi libraries and, uh, free up the, the time of their personnel in order to cover other projects while the canvas is, uh, doing its work. So on the left, you see how that works and their operation flow chart and how the mural canvas help consolidate a lot of the touchpoint steps that are highlighted by the, the hand, uh, icon and how that freed up time for their personnel to cover other projects. Uh, so notice how much fewer these are. And on the right, the results are actually very comparable with a left figure, uh, indicating box plots, uh, and actually tighter spread of, of the obtained library yields. And on the right, the quality scores, uh, from the sequencing runs, uh, actually comparing really well to mural canvas, uh, to manual prep preparation. Now, the, the broad actually mentioned that in, in their evaluation, but they observe this technology is as good as manual. And in terms of turnaround time, reproducibility and data quality is an excellent option. Uh, for, for lab automation, for nanopore sequencing, we actually here work to reduce, uh, uh, significantly the, the reaction volumes for this automation protocol. And we operate at one fourth of, uh, what the kid is offered, and we're able to generate libraries using one microgram of microbial community, high molecular weight, d n a standard. Um, this is standard offer from xmo. Here you see the, uh, redistribution plots from preparing this manually or using the mural canvas, and you see how comparable they look, uh, with, with, um, from onto another. And then in the next table, um, first notice the N 50 score, and then of course, the, um, read length and the quality of, of these prepared libraries that are within the same range to, to manual when comparing to MI canvas. Finally, in this table, this specific mock d n a standard contains, uh, distribution of, uh, bacteria and one GE species, all of which are identified in the MI canvas prepared library, uh, in similar proportions as, uh, they were in the manual preparation. And for this protocol, one of our customers, the A Airline Institute, uh, used the MI canvas to prepare nanopore genomic libraries, uh, to sequence a plan genome in this particular example. And another thing they did different was to challenge a system by reducing the input amount all the way down to 250 nanograms for this particular application, uh, of abido strana, D n a. So on the right, you can see in the, in the table, the N 50 scores of the automated runs, uh, compare really well to the manual, uh, run. And then, uh, on the top table. And then on the bottom table, the actual number of, uh, s needed to assemble this genome, uh, being very similar from one method to another, um, independent to what bio formatic tool is used for, uh, doing that. Now, in a collaboration with an expert lab in the field, we were able to, in ultra lung sequencing, we were able to, um, modify the, the nano genomic d n a, uh, by ligation kit, uh, in the miam, and actually develop a script that enables ultra long, uh, read d n A sequencing. And here you see the, the redistribution plot from a mural canvas prep library, and, uh, that use this modified script and how it compares to the manual, uh, prepared library. For this particular example, we used really high quality d n a extracted from, uh, cell lines HEC 2 9 3 cells, and we treated the, um, extracted genomic d n a high molecular wave d n a with a short read eliminator, uh, that kept, kept all of the fragments above 25 kb. So the N 50 score that was achievable through this method. And New York Canvas automation reached all the way up to 77 kb. And then one of our customers, uh, the McGill Genome Center used these modified automations script on your canvas for their specific application to automate ultra long, uh, d n a sequencing of challenging human clinical samples. And on the top, you see the flow, their flow chart of how the clinical sample whole blood sample gets processed, all the way to sequencing. It also goes through short read elimination. And with that, they were able to obtain, um, and 50 scores from their sequencing runs at average, at about 40 kb, which in a clinical setting, uh, provided high quality data, allowing for, uh, single nucleotide variant detection, as well as a very large deletion of the Titan gene. So on the left, uh, integrated, integrated, uh, genome viewer snapshot, you see the single nucleotide variant detection for Titan gene in one patient on the right, uh, you see how the very large deletion is visible in the same I G B, uh, snapshot of a different patient. And so this type of analysis and variant characterization could not be possible without, um, long read sequencing. And here the mural canvas was able to match the coverage characteristics and sequencing metrics of the manual preparation. And for this customer, it was able to provide an option for a rapid, uh, sample characterization in the clinical research setting using long read sequencing as a, as a method. Uh, instead now we can take a look at also some, uh, results from whole genome sequencing using short read sequencing. And here we have actually worked with the latest Illumina kit, the, the Illumina D N A prep PCR pre kit to demonstrate the applicability of MI canvas for rapid turnaround whole genome sequencing, um, and particularly the handling of, uh, urgent samples, which require extra caution, uh, because we must ensure the, the best quality of the, of the sequencing results in the clinical setting. So across three different input amounts, uh, that were used of NA 12 8 7 8 as a d n a reference standard that range from 500 nanogram all the way to 50 nanogram, the MI candle libraries showed quality key sequencing metrics that are presented in this table. And the, for this application, variant detection is the goal. So all of the data were sub-sample to four tx, uh, sequencing coverage. And then the neuro canvas libraries were showing equal or better F one, uh, scores for both single nucleotide variants as well as insertion deletions. And then the percent of base is covered at 20 x, uh, uh, was the same or very similar across all of the input ranges of the tested BI material. Now here, the pioneering lab in, in rapid turnaround whole genome sequencing at Radi Children's Hospital, uh, used the MI canvas for the processing of, of genomic D N A that comes from critically ill children and their parents, uh, what is called TRIO family sequencing. So the, the canvas was able to deliver high quality libraries for rapid whole genome sequencing. And the input amount used here from this clinical sample was at 500 nanogram, and the sequencing was performed using the Illumina NovaSeq 6,000. Now, the sequencing metrics and the variant calling, uh, including the structural variants the lab was interested in, met their internal passing criteria. And on the right slide, uh, on the right of the slide, you can actually see the, the two of of the trio families that were examined. These were already characterized samples where they knew what they were looking for, and they were testing the MI canvas, uh, to confirm it can reliably, uh, identify this variant. So it was possible to identify, uh, a single nucleotide variant and a deletion in the first family, which is on the top, uh, integrated genome view. Um, and in the bottom, there were two single nucleotide variants for the second family trio. And the I G V snapshots that you see here help the seer that each of the variants reported in the critically ill, uh, infants were actually inherited, uh, from the respective mother and father, uh, in both of the, of the family trio. So for this application, we're thinking we're, we're talking about minimal hands-on time that you see on the left of the slide, uh, how it's all now boiling down to preparing the, the, the cartridge loading it and running the, the sample. Um, the MI canvas system provided this lab an alternative, uh, for ultra rapid whole genome sequencing of critically ill children where batching and staffing is not always a viable option in, in the clinical setting. So it's a, it's a very important, uh, and, and, and relevant application for this type of setting using the milk bundle. Now, let's review the results generated from whole exome sequencing. So here, these type of protocols are the longer ones. So they, they, they last about five and a half hours. Um, not only their TED and and multi-step, they also involve P C R reactions with, in some cases, depending on the size of the panel, uh, reaching up to 15 P c r cycles. And that is something the MI canvas has been designed to do, uh, really well, same way as a P C R cycle. So for this application, we use the, the twist, human core exome, past hybridization target enrichment kit, uh, to capture the individual libraries. In this particular slide that you see from NA 12, 8 7 8 genomic D N a and the input here was 500 nanograms. That's what was taken into hybridization capture, uh, protocol and the bar charts. Uh, you can see the mean value of four replicate runs of either manual, which is depicted in gray bar charts, or, uh, mural canvas depicted in blue. So you can see how mural canvas was able to yield equivalent target enrichment, uh, sequencing metrics. Uh, we, we have here the most important ones, uh, such as the percent, uh, of duplicates, uh, the percent on target, the fold eight score, and on the very right, the depth of coverage, both at 20 x and also at 30 x for this protocol, achieving these results in a fully automated fashion and such a seamless, uh, loading is, is, uh, really game changing for labs that just simply lack the expertise or have technicians that are overwhelmed with many different protocols running in parallel 'cause in the duration of five and a half hours, it is not impossible for something to go wrong. And these are precious samples and long work. Now, we repeated this experiment, but this time around, uh, enriching a pool of libraries instead of an individual library all at the same cartridge. And we compared, again, mural canvas to manual the input amount for this experiment. What we took through enrichment and capture was, uh, one, one and a half micrograms of, uh, D N A from all of the pool samples. And here, too, we could obtain, as you can see on the on the table, um, target enrichment sequencing matrix that look comparable to the manual run when, for especially clinical applications visualizing the, the read that CFI map to the exome targeted regions, it is possible to, as shown in this example of the E G F R gene, specifically, it's exome two to 10, uh, it is possible for the neuro canvas to deliver confident variant choline. And if you focus in exon four, uh, that is a heterozygous variant example that is, uh, comparing the two top manual tracks of I G B and the two bottom, um, um, automated tracks of neuro canvas, uh, detecting both as part of a large reliability study, uh, that was conducted with the MI canvas to examine inter operator and intersystem variability and the impact it may have on, on key target enrichment metrics. Um, we generated, uh, these results from multiple runs. And here you're see box dots, the 30 x coverage and fold AP score values from your canvas in blue dots, uh, of the box plots, and then from, for manual libraries in black. So comparing the two, you see a similar range, uh, where the, the automated runs are confined, and this is now with several, uh, eight different operators and tens of instruments involved in this study. So ensuring reliable performance across hands and across instruments. We have gone through, uh, quite a, a few results, uh, from the ocam. So I would like to recap now, um, we have several protocols available and ready to go for both whole genome sequencing and for poly exome sequencing, both for long read sequencing and short read sequencing. Um, we reviewed together reproducible Next generation sequencing results that demonstrate high quality performance of neuro canvas prepared libraries. And then we have a number of applications that actually reduce significantly the amount of reagents using your existing current providers. Uh, lastly, the automation solution allows you to edit and switch protocols seamlessly without expert training or expert operators. And in the process of using the mural canvas, uh, since everything is consolidated in the consumable, uh, electronics free plastic cartridge, there is fewer tips, uh, used of course, because they're only used for loading the, the inch, the cartridge at the start of the round. So thank you for listening. Uh, we would love to hear from you and to help you introduce the mural canvas in your daily lab operations for either long read or short read sequencing. So feel free to reach out for that and, um, looking forward to hearing from you. Alright, great. Thanks very much Faye for a wonderful presentation. So at this point, we are ready to move into our q and a session with the audience. Again, for those of you who may have joined us late, you can send in your questions by typing 'em into the q and a box on the right hand side of your screen. Even if you don't have a question, let us know how you enjoyed this presentation, if you found the information useful, and if you'd like us to pass your info along to Faye and the Integra team. Another reminder to please visit the handout section on the right hand side of side of your screen for supporting information for this event. There's also a certificate of completion for this webinar if you need it, and I'd like to remind you to please, uh, answer our poll question if you haven't already, for a chance to win a gift card courtesy of lab manager. Uh, so Faye, thanks again. Uh, if we'd like to start with the q and a from the audience, we have our first question here. It says, based on the, um, excuse me, I'm sorry. Uh, it says, what is the lowest volume that the system can handle? Uh, sorry, I'm just unmuted. The lowest volume the system can handle is, uh, what we pipe it into the cartridge, uh, as minimum volume is three, three and a half, 3.3 microliters and, and the highest range is all the way up to, uh, 200 microliters. So it's, it's a wide range. Okay, great. Thank you. Uh, underneath that we have another question from the audience. This one says, during thermocycling, inside the cartridge, do small volume droplets experience any evaporation? Uh, we have mitigated for that, obviously, because the, uh, matrix of digital microfluidic approach we deployed, uh, is air. So we have a system reagent that is proprietary that mitigates evaporation and, and gets combined, uh, especially with, uh, lower volume master mixes. Uh, so the evaporation is, is trivial, it's, it's, um, negligible and does not affect the functional, um, readout. All right, wonderful, thank you. Um, we have some more questions coming in from the audience. If you go to the top of your list, we have another one here. It says, based on a list of protocols developed for the Miro canvas, how did you decide to develop these protocols? Right. We, we go with the, the ones that are most asked for. And, uh, in, in this process of, um, protocol, uh, release and development and release, uh, we, we really like to stay in, in close contact with our customers or potential customers and hear what these, uh, their daily operation needs, uh, most burning daily operation need and, uh, tailor our, our product roadmap, uh, around these in order to release, um, solutions that will actually be used. Okay, wonderful. Thank you. Our next question says, what about the system makes it well suited for long read sequencing? Um, I mentioned in, uh, in previous slides how the diffusive, uh, gentle, uh, forces of electrowetting when mobilizing an, uh, a droplet, as I said by actuating, uh, unique electrodes, uh, are, are allowing the migration of, of the droplet with the d n A to be gentle and and smooth through the processing steps of library preparation. So by doing that, uh, we maintain the molecule intact, and of course, that has an advantage when it comes to the longest possible reads, uh, being sequenced out out of the library. All right, wonderful. Thank you. The next question says, what is the highest temperature the instrument can reach? Right, the, I mean, P C R cycling is, uh, demanding with, with high temperatures. We, we, we cover, uh, standard protocols that require up to 98, uh, degrees Celsius. The, the instrument can, can heat up to more than a hundred, but of course, we don't want to boil the, the sample. So yes, the maximum would be 98 for for P c R steps. Thank you. Uh, this question says, is the system capable of R n a sequencing short read library prep? Uh, yes. We, we have actually demonstrated that, uh, we haven't yet released, uh, uh, a supported protocol. Uh, but it is definitely in our roadmap and it is definitely possible, uh, both for short read rna, ic and for long read RNA sic. Great. Thank you. This one says, how long of fragments do you think you could work with? What about really long fragments? Right. The, the results I shared with this, um, modified, um, automation script we have for ultra long, uh, d n A sequencing, the maximum we have reached so far in our lab is, uh, uh, 77 KB and 50 score. And, uh, it, it really boils down to the expertise of the lab to handle the input d n A material. So our lab is not at that level as, as, um, academic labs that have, uh, special extraction protocols and handling methods to really push the, the limit of that. So we, we are very e excited to, uh, have the miam tested in, in hands of, uh, such expert labs and see if, if the N 50 score can go even higher. Theoretically, uh, with the protocol modifications we have, it should be able to, to reach the highest possible N 50 score. Okay, great. Thanks. We have time for a couple more questions. This one says, how can I modify protocol scripts if I wanna do additional P C R cycles? Can I add them? Yes, there is a, um, together with a physical product, uh, the mural canvas and the cartridges needed, there is a web application where you can, uh, visualize all of the steps in the protocol and there are, um, sections where you can modify the number of P C R cycles or the temperature. Of course, everything is within, uh, some, some ranges that we have predetermined, uh, but there is some degree of flexibility to increase P C R or decrease P C R cycles and, uh, determine duration and temperatures. Okay, great. Thanks. We have time for one last question. This one says, are there limitations on the D n a input amount and quality of sample? We typically follow the, when we develop a new protocol, we work with the minimum recommended input amount that the kit, uh, is released with so from the vendor of, of the corresponding kit. And so that's, that's the minimum we, we have verified when we release the product. Again, um, very curious to, to have it tested in the customer's hands if this is something they want to explore and make new claims, uh, to go below what, what the key vendors promise. Alright, wonderful. Thanks so much. So that does bring us to the end of this webinar. And on behalf of Lab Manager, I'd like to thank Dr. Fay Christodoulou for all the hard work she put into this presentation. And I'd like to thank all of you for taking time outta your busy schedules to join us. A reminder that all of the presentations in the Automation Digital Summit will be available for free on-demand viewing. Please watch your email for a message from Lab Manager once these videos become available. Once again, thank you to our sponsors, Integra Biosciences Brand Tech, scientific Metro U s A analytic, Jenna Comp type, and Automata. Their support allows Lab Manager to keep these events free of charge for our readers. Please be sure to tune in for our next presentation at 2:00 PM Eastern today, connecting multiple labs and or experiments unifying science on lab automation level. For more information on all of our upcoming or on-demand webinars, or to learn more about the latest tools and technologies for the laboratory, please visit our website@labmanager.com . Thank you to all of you for being part of our Automation Digital Summit, and we hope you have a great day.
