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Automation and Consistency in PBMC Separation

Get high-throughput PBMC separation that rivals manual methods

There are several manual methods to separate peripheral blood mononuclear cells (PBMCs) from leukapheresis products, but most of them are highly user-dependent and laborious.  The Gibco™ CTS™ Rotea™ Counterflow Centrifugation System can be used to automate a variety of methods to separate PBMCs in a closed environment.

In this 12-page app note, you’ll see effective and consistent PBMC separation across all tested method compared to a manual density gradient centrifugation method.

Test methods include: 

Guarantee Consistency in Your PBMC Separation
  • Ficoll solution
  • With ACK (ammonium-chloride-potassium) lysis buffer
  • Without lysis buffer

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