There are several manual methods to separate peripheral blood mononuclear cells (PBMCs) from leukapheresis products, but most of them are highly user-dependent and laborious. The Gibco™ CTS™ Rotea™ Counterflow Centrifugation System can be used to automate a variety of methods to separate PBMCs in a closed environment.
In this 12-page app note, you’ll see effective and consistent PBMC separation across all tested method compared to a manual density gradient centrifugation method.
Test methods include:
- Ficoll solution
- With ACK (ammonium-chloride-potassium) lysis buffer
- Without lysis buffer
