Top 5 Signs That You Should Service or Replace Your HPLC Columns
![]() | The backpressure increases above the column or system limit. A common practical limit is a 10-20% increase. |
![]() | A quantitative chromatographic system suitability parameter fails: resolution, tailing factor, S/N, RRT, efficiency. |
![]() | A qualitative chromatographic parameter changes significantly from the expected: Peak shape, background response, irregular baseline disturbances, column leaking. |
![]() | Visible external hardware wear and tear: inlet/outlet threading wear, corrosion, reagent precipitates (buffer salts or derivatizing agents). |
![]() | Known exposure to components that could afflict future selectivity under new conditions: ion-pairing agents, sample contaminants (such as inorganic ions or phospholipids), phthalates, immiscible solvents. |
Top 7 Questions That You Should Ask When Buying HPLC Columns
![]() | Based on your analyte(s), matrix, separation goals, and instrumentation, what column does the vendor recommend? |
![]() | What benefits does this column offer over your current column? Performance? Lifetime? Reproducibility? Etc. |
![]() | How should you clean/prepare your sample prior to injection on the column? |
![]() | How do you care for the column? Conditioning, cleaning, storage, etc. |
![]() | What type of chromatographic media (fully porous, monolithic, core-shell) is going to provide the most benefit for your separation? |
![]() | Do you need a unique selectivity (HILIC, polar-end capped, etc.) to separate any very polar and/or non-polar components in your mixture? |
![]() | What column dimension is going to be most suitable for your loading requirement? |
Recently Released HPLC Columns
CORTECS UPLC Columns
-
Allow laboratories to produce greater amounts of information faster with every chromatographic separation
- Available in C18, C18+ or HILIC chemistries and offered in 30 unique column configurations available for shipping worldwide
- Column particles feature a solid, impermeable silica core encased in a porous silica outer layer where the interactions between the stationary phase and the analytes occur
Waters
www.waters.com
Kinetex® Preparative HPLC and SFC Core-Shell Media
-
Kinetex 5-micron particles in four phases—C18, XB-C18, Phenyl-Hexyl and PFP—are now offered in 21.2 mm ID Axia packing
- Axia preparative format delivers longer column lifetime, higher efficiencies, improved performance and high reproducibility, compared to conventionally packed columns for lab-scale prep chromatography
- Axia packing eliminates the drawbacks of traditional slurry packing, delivering uniform bed density and dramatic performance advances
Phenomenex
www.phenomenex.com
MAbPac Protein A HPLC Columns
-
For high-performance monoclonal antibody titer analysis
- Specially designed for higher sensitivity and accurate concentration analysis over a wide linear range to minimize the need to re-analyze samples
- Novel non-porous polymeric resin with hydrophilic surface offers fast mass transfer to enhance throughput and minimize carryover
- Accommodate high flow rates to further increase throughput
Thermo Fisher Scientific
www.thermoscientific.com
TSKgel® SW mAb Size Exclusion HPLC Columns
- Meet the growing demand for the higher resolution separation of mAb monomer and dimer/fragment, as well as higher resolution of mAb aggregates
- Offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates
- Include the TSKgel SuperSW mAb HR and TSKgel UltraSW Aggregate
Tosoh Bioscience
www.tosohbioscience.com
HPLC Column Manufacturers
Advanced Chromatography Technologies | www.ace-hplc.com |
| |
Agela Technologies | www.agela.com |
| |
Agilent | www.agilent.com |
| |
Applied Biosystems | www.appliedbiosystems.com |
| |
Beckman Coulter | www.beckmancoulter.com |
| |
Bio-Rad | www.bio-rad.com |
| |
Biotage | www.biotage.com |
| |
ChiralTech | www.chiraltech.com |
| |
Dynamax | www.dynamaxcorp.com |
| |
Eksigent | www.eksigent.com |
| |
ES Industries | www.esind.com |
| |
Grace Discovery Sciences | www.discoverysciences.com |
| |
Hamilton | www.hamiltoncompany.com |
| |
Imtakt | www.imtaktusa.com |
| |
J & K Scientific | www.jkscientific.com |
| |
MAC-MOD | www.mac-mod.com |
| |
MicroSolve | www.microsovetech.com |
| |
Peeke Scientific | www.peekescientific.com |
| |
PerkinElmer | www.perkinelmer.com |
| |
Phenomenex | www.phenomenex.com |
| |
Proxeon | www.proxeon.com |
| |
Restek | www.restek.com |
| |
Shiseido | www.shiseido.com |
| |
Shodex | www.shodex.com |
| |
SiliCycle Inc. | www.silicycle.com |
| |
Supelco | www.sial.com |
| |
Thermo Fisher Scientific | www.thermofisher.com |
| |
Valco | www.vici.com |
| |
Waters | www.waters.com |
| |
Whatman | www.whatman.com |
| |
Zirchrom | www.zirchrom.com |
| |
Tosoh Bioscience | www.tosohbioscience.com |
|