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HPLC Sample Prep

Find out all you need to know when buying SPE equipment for your lab and the results of our most recent sample prep survey.

by Lab Manager

Top 6 Questions You Should Ask When Buying HPLC Sample Prep Products (specifically, SPE equipment):

1. What is your sample matrix?

3. What are your target analyte(s)?

4. What are your sample clean up goals (high recovery vs. clean-up vs. speed)?

5. What sample preparation format is best for your work?

6. What is your mode of analysis (LC/UV, LC/MS/MS, GC/FID, GC/MS, etc.)?

Close to 40 percent of respondents to our latest sample prep survey currently automate or are planning to automate their entire sample preparation process or specific techniques within the process.

Yes 14%
No, but planning to automate the process 24%
No, and no plans to automate 62%

Sample preparation can be achieved using several different techniques, and sometimes the best technique is dictated by the sample type. Based on respondents’ replies, the most common techniques include filtration, solvent extraction, liquid- liquid extraction (LLE) and solid phase extraction (SPE).

Filtration 20%
Solvent Extraction 18%
Liquid-Liquid Extraction (LLE) 15%
Solid Phase Extraction (SPE) 14%
Protein Precipitation (PPT) 7%
Dialysis 4%
Solid Phase Micro Extraction (SPME) 4%
Immunoaffinity Extraction (IAE) 3%
Matrix Solid Phase Dispersion (MSPD) 2%
Supercritical Fluid Extraction (SFE) 2%
Other 11%

The top three features / factors that influence the decisionmaking process for making improvements in sample preparation are: Lower costs (reagents, syringe filters, vials and other related accessories)—97 percent; greater quality of data—94 percent; and faster sample prep time—85 percent.

Lower cost (less solvent, reagent consumption, etc…) 97%
Greater accuracy/improve quality of data 94%
Faster sample prep time (processing multiple samples) 85%
Greater recoveries—minimal sample transfer 50%
Easier data review 44%
Improved safety (reduced solvent/sample exposure and glassware) 39%
Making an investment in instruments 38%
Other 6%

Sample preparation is an essential step in many analyses and the importance of accurate sample preparation techniques cannot be overstated. The sample preparation procedure significantly impacts assay throughput, data quality, and analysis cost. Contamination may affect the analytical results for all applications, hence, reagents used in sample preparation, including water, need to be of increasingly high purity. Based on vendor estimates, errors of commonly used measurement methods are usually below 20 percent but can easily rise to more than 200 percent. To avoid the total failure of an analytical procedure, it is better to invest effort and money in the improvement of sample preparation techniques rather than squeezing the measurement procedures to get one or two percent improvement in standard deviation.

Recently Released Sample prep products

Strata™-X Polymeric SPE Sorbents
• Provide unique polymeric selectivities that cover a diverse spectrum of analytes
• Simplify the method development process for fast and efficient sample preparation
• Retains analytes by several different sorbentanalyte interactions
• Remove unwanted contaminants including phospholipids for excellent chromatography
• Large-particle, large-pore options are also available


Samplicity® Filtration System
• Designed to provide a high-throughput alternative to syringe filters when preparing samples for UHPLC and HPLC analysis
• Enables simultaneous filtration of up to 8 samples in seconds
• Low hold-up volume allows processing of samples as small as 300 μL
• Available with Millex Samplicity® filters made with EMD Millipore’s hydrophilic polytetrafluoroethylene (PTFE) membranes

EMD Millipore

SiliaPrepX™ Polymeric SPE cartridges & Well Plates
• Products include all the phases currently used in the field of sample treatment (HLB, DVB, SCX, SAX, WCX and WAX)
• Presents an excellent lot-to-lot reproducibility allowing clean extract, reducing the ion suppression and increasing the selectivity for LC-MS/MS applications
• Complete range of sorbents has a high loading capacity


GX ASPEC™ Series
• Provides rugged and reliable automation of Solid Phase Extraction (SPE) applications utilizing 1,3 or 6 mL cartridges
• Utilizes Mobile Rack technology to completely automate the SPE process
• On-bed reservoirs allow access of up to four solvents without taking up a valuable rack position
• Features Jet Wash rinse technology with optional rinse pump


HPLC Sample Prep Product Manufacturers

BDH Chemicals
Horizon Technology
Perfinity Biosciences
Porvair Sciences
Thermo Fisher Scientific